Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001358
UniProt IDP52272
Primary gene name(s)HNRNPM
Synonym gene name(s)HNRPM, NAGR1
Protein nameHeterogeneous nuclear ribonucleoprotein M
Protein functionPre-mRNA binding protein in vivo, binds avidly to poly(G and poly(U RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
Subcellular locationNucleus, nucleolus {ECO:0000269|Ref.5}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P52272
Gene Ontology
(Biological Process)
Complete annatation
alternative mRNA splicing, via spliceosome [GO:0000380];
fibroblast growth factor receptor signaling pathway [GO:0008543];
gene expression [GO:0010467];
mRNA splicing, via spliceosome [GO:0000398]
Gene Ontology
(Molecular Function)
Complete annatation
nucleotide binding [GO:0000166];
poly(A RNA binding [GO:0044822];
protein domain specific binding [GO:0019904];
RNA binding [GO:0003723]
Gene Ontology
(Cellular Component)
Complete annatation
catalytic step 2 spliceosome [GO:0071013];
cell surface [GO:0009986];
extracellular exosome [GO:0070062];
extracellular matrix [GO:0031012];
integral component of plasma membrane [GO:0005887];
membrane [GO:0016020];
nuclear matrix [GO:0016363];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
paraspeckles [GO:0042382];
spliceosomal complex [GO:0005681]
Protein-protein interaction110751
Phylogenetic treeP52272
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9110726244071990.005694940598245380.016173760070992
AZA vs. DISU-0.4121212528380270.1074907726194070.653579050610225
AZA vs. IL70.2485732799030190.1949520833991780.958003422925385
AZA vs. SAHA-0.3093827328082880.2043096287134270.572035079910209
DISU vs. CD3-1.33430377400630.0002959287775986530.00145826426725142
DISU vs. IL70.650876594757010.01005616121217880.117910554267693
DISU vs. SAHA0.1045759224432060.7192152976171250.917103213595783
DMSO vs. AZA-0.09307202579344850.6541734168647651
DMSO vs. CD3-1.009484080761280.001736492027714980.00550626234424881
DMSO vs. DISU0.3187964623845430.2721388983388160.79139779916016
DMSO vs. IL70.3481574117960320.07544560005711560.519571686094572
DMSO vs. SAHA-0.2241104324524840.3836131914852980.73068630966836
HIV vs. Mock in Activation0.2810812565567330.6518728614776830.999983755607037
HIV vs. Mock in Latency-0.04945346067015270.7644705146690250.999834320637052
IL7 vs. CD3-0.6563849172440290.04143556666059230.0940831957529164
SAHA vs. CD3-1.243786938199480.0005202364310183950.00202269427643117
SAHA vs. IL7-0.5602613121350630.02169483949418820.105800068112388
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.386962 0.00199466
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.018 1.028 0.96 0.969 1.068
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2DGV NMR - A=652-730.
2DH9 NMR - A=655-730.
2DO0 NMR - A=196-296.
2OT8 X-ray 3.1Å C/D=41-70.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) incorporates 25631074
Rev interacts with 22174317
integrase interacts with 24968937
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Pr55(Gag) complexes with 23125841
Tat interacts with 25496916
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03040 Spliceosome - Homo sapiens (human)