Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001352
UniProt IDP07910
Primary gene name(s)HNRNPC
Synonym gene name(s)HNRPC
Protein nameHeterogeneous nuclear ribonucleoproteins C1/C2
Protein functionBinds pre-mRNA and nucleates the assembly of 40S hnRNP particles, PubMed:8264621. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules, PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides, PubMed:8264621. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine, m6A has been shown to alter the local structure in mRNAs and long non-coding RNAs, lncRNAs via a mechanism named 'm(6A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing, PubMed:25719671. {ECO:0000269|PubMed:12509468, ECO:0000269|PubMed:16010978, ECO:0000269|PubMed:25719671, ECO:0000269|PubMed:7567451, ECO:0000269|PubMed:8264621}.
Subcellular locationNucleus. Note=Component of ribonucleosomes.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P07910
Gene Ontology
(Biological Process)
Complete annatation
3'-UTR-mediated mRNA stabilization [GO:0070935];
ATP-dependent chromatin remodeling [GO:0043044];
gene expression [GO:0010467];
mRNA splicing, via spliceosome [GO:0000398];
negative regulation of telomere maintenance via telomerase [GO:0032211];
osteoblast differentiation [GO:0001649];
protein sumoylation [GO:0016925];
RNA splicing [GO:0008380]
Gene Ontology
(Molecular Function)
Complete annatation
identical protein binding [GO:0042802];
mRNA 3'-UTR binding [GO:0003730];
N6-methyladenosine-containing RNA binding [GO:1990247];
nucleosomal DNA binding [GO:0031492];
nucleotide binding [GO:0000166];
poly(A RNA binding [GO:0044822];
poly(U RNA binding [GO:0008266];
RNA binding [GO:0003723];
RNA polymerase II core promoter proximal region sequence-specific DNA binding [GO:0000978];
RNA polymerase II distal enhancer sequence-specific DNA binding [GO:0000980];
telomerase RNA binding [GO:0070034]
Gene Ontology
(Cellular Component)
Complete annatation
catalytic step 2 spliceosome [GO:0071013];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
membrane [GO:0016020];
nuclear chromatin [GO:0000790];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
pronucleus [GO:0045120];
protein complex [GO:0043234];
spliceosomal complex [GO:0005681]
Protein-protein interaction109424
Phylogenetic treeP07910
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.598003032532971.83613743698352e-061.34602448687819e-05
AZA vs. DISU0.1082661401214590.6680400910744120.962557690454146
AZA vs. IL70.1843261375706710.3358685494389580.999311006273513
AZA vs. SAHA-0.2967065849658930.2235904120850080.591933474124096
DISU vs. CD3-1.502821321509425.06105604127782e-050.00031240933155701
DISU vs. IL70.06714182145414080.7894942031699320.951957938107586
DISU vs. SAHA-0.4038061740997310.1673436492279280.528265486061943
DMSO vs. AZA-0.02599189915482080.8760724395777921
DMSO vs. CD3-1.635652953013145.89769204917268e-074.4269422382056e-06
DMSO vs. DISU-0.136245426013250.5758646569727660.931573613915837
DMSO vs. IL70.2176192864585090.2244271652830640.731490098375259
DMSO vs. SAHA-0.2777303392999890.2384524784975110.586738642698405
HIV vs. Mock in Activation-0.0009736845541419660.9987492427216050.999983755607037
HIV vs. Mock in Latency-0.01590464355095980.9228620944946670.999834320637052
IL7 vs. CD3-1.4055117198441.70924730981126e-050.000123684516086342
SAHA vs. CD3-1.919568363221531.41497103811616e-071.4196813278569e-06
SAHA vs. IL7-0.4847233517822340.04668658592864050.175455895359842
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.165573 0.409407
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.042 0.995 1.013 0.971 1.007
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1TXP NMR - A/B/C/D=194-220.
1WF2 NMR - A=8-92.
2MXY NMR - A=2-106.
2MZ1 NMR - A=2-106.
3LN4 X-ray 1.3Å C=102-117.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 22174317
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Pr55(Gag) complexes with 23125841
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03040 Spliceosome - Homo sapiens (human)