Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001336
UniProt IDP08397
Primary gene name(s)HMBS
Synonym gene name(s)PBGD, UPS
Protein namePorphobilinogen deaminase
Protein functionTetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
Subcellular locationCytoplasm {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P08397
Gene Ontology
(Biological Process)
Complete annatation
heme biosynthetic process [GO:0006783];
peptidyl-pyrromethane cofactor linkage [GO:0018160];
protoporphyrinogen IX biosynthetic process [GO:0006782]
Gene Ontology
(Molecular Function)
Complete annatation
amine binding [GO:0043176];
carboxylic acid binding [GO:0031406];
coenzyme binding [GO:0050662];
hydroxymethylbilane synthase activity [GO:0004418];
uroporphyrinogen-III synthase activity [GO:0004852]
Gene Ontology
(Cellular Component)
Complete annatation
axon [GO:0030424];
condensed chromosome [GO:0000793];
cytosol [GO:0005829];
nucleus [GO:0005634]
Protein-protein interaction109388
Phylogenetic treeP08397
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.308197627503929.51481099444873e-050.000461710339048072
AZA vs. DISU-0.4043532505560320.1422472364150830.717798021069077
AZA vs. IL70.527774087023930.007079998913386950.27187078318306
AZA vs. SAHA-0.3912734233003520.1149482173163210.432416651760292
DISU vs. CD3-1.725033180934676.1029850343397e-064.80901183521363e-05
DISU vs. IL70.922982488561640.001518308435922490.0362098957137686
DISU vs. SAHA0.01476385044796470.9605515500017640.99168431881472
DMSO vs. AZA0.1025655094073360.5513505479415521
DMSO vs. CD3-1.220009296196970.0001862775995373770.000780170152079672
DMSO vs. DISU0.504142277258130.06640978732623140.485734688085979
DMSO vs. IL70.4331511512905910.01798923312875370.27871293876255
DMSO vs. SAHA-0.4992371444945810.03733483394245770.211690474923544
HIV vs. Mock in Activation-0.2726535402018340.6619563971254070.999983755607037
HIV vs. Mock in Latency-0.2886742499692110.09018704354592640.999834320637052
IL7 vs. CD3-0.7727332756810640.01755288899926480.0472440440932485
SAHA vs. CD3-1.724300801631392.25803436293415e-061.68541742691519e-05
SAHA vs. IL7-0.9226969278332360.0002039051735442940.00340438752086392
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.387969 0.00864647
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.03 0.979 0.963 0.948 0.963
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3ECR X-ray 2.1Å A/B=1-361.
3EQ1 X-ray 2.8Å A/B=1-361.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00860 Porphyrin and chlorophyll metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)