Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001296
UniProt IDP52789
Primary gene name(s)HK2
Synonym gene name(s)unknown
Protein nameHexokinase-2
Protein functionunknown
Subcellular locationMitochondrion outer membrane {ECO:0000250}. Note=Its hydrophobic N-terminal sequence may be involved in membrane binding. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P52789
Gene Ontology
(Biological Process)
Complete annatation
apoptotic mitochondrial changes [GO:0008637];
canonical glycolysis [GO:0061621];
cellular glucose homeostasis [GO:0001678];
establishment of protein localization to mitochondrion [GO:0072655];
glucose transport [GO:0015758];
glycolytic process [GO:0006096];
lactation [GO:0007595];
maintenance of protein location in mitochondrion [GO:0072656];
negative regulation of mitochondrial membrane permeability [GO:0035795];
negative regulation of reactive oxygen species metabolic process [GO:2000378];
positive regulation of mitophagy in response to mitochondrial depolarization [GO:1904925];
regulation of glucose import [GO:0046324];
response to ischemia [GO:0002931]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
fructokinase activity [GO:0008865];
glucokinase activity [GO:0004340];
glucose binding [GO:0005536];
hexokinase activity [GO:0004396];
mannokinase activity [GO:0019158]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
membrane [GO:0016020];
mitochondrial outer membrane [GO:0005741]
Protein-protein interaction109346
Phylogenetic treeP52789
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7621565801629740.02118330634932590.048841179944476
AZA vs. DISU-1.267464984975790.002352679686256960.121870424710094
AZA vs. IL70.4079968736794230.0345699575561120.571351941056224
AZA vs. SAHA-0.3246353556694960.1853909176323190.550114703226296
DISU vs. CD3-2.040333641903495.90508274689316e-064.66770935116243e-05
DISU vs. IL71.666109512468820.0001175388895588990.00590632920033468
DISU vs. SAHA0.9440498915849730.02220364585752180.173294224090477
DMSO vs. AZA0.08045951040217950.6327489658331971
DMSO vs. CD3-0.6911192023218390.03176145316010940.0658165463352198
DMSO vs. DISU1.346586077571360.001158937647010470.0690323728871455
DMSO vs. IL70.3348292670263140.06377152178815850.484139846147069
DMSO vs. SAHA-0.4116349117513910.08201639674021110.334072733857222
HIV vs. Mock in Activation0.3456677010817760.5798680177972120.999983755607037
HIV vs. Mock in Latency0.1397653733763130.6417126769507530.999834320637052
IL7 vs. CD3-0.3474722022264920.2829480478071110.419317267190697
SAHA vs. CD3-1.110288409848960.001904692190601190.00621785201240996
SAHA vs. IL7-0.7355093287108020.002769121232353840.0250100260374487
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.3346 0.04998

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.470449 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.032 0.888 1.046 0.895 0.881
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2NZT X-ray 2.4Å A/B=17-916.
5HEX X-ray 2.7Å A/B=17-917.
5HFU X-ray 2.9Å A/B=17-917.
5HG1 X-ray 2.7Å A=17-916.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 22174317
Vpr upregulates 23874603

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00010 Glycolysis / Gluconeogenesis - Homo sapiens (human)
hsa00051 Fructose and mannose metabolism - Homo sapiens (human)
hsa00052 Galactose metabolism - Homo sapiens (human)
hsa00500 Starch and sucrose metabolism - Homo sapiens (human)
hsa00520 Amino sugar and nucleotide sugar metabolism - Homo sapiens (human)
hsa00524 Neomycin# kanamycin and gentamicin biosynthesis - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01200 Carbon metabolism - Homo sapiens (human)
hsa04066 HIF-1 signaling pathway - Homo sapiens (human)
hsa04910 Insulin signaling pathway - Homo sapiens (human)
hsa04930 Type II diabetes mellitus - Homo sapiens (human)
hsa04973 Carbohydrate digestion and absorption - Homo sapiens (human)
hsa05230 Central carbon metabolism in cancer - Homo sapiens (human)