Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001292
UniProt IDP15822
Primary gene name(s)HIVEP1
Synonym gene name(s)ZNF40
Protein nameZinc finger protein 40
Protein functionThis protein specifically binds to the DNA sequence 5'-GGGACTTTCC-3' which is found in the enhancer elements of numerous viral promoters such as those of SV40, CMV, or HIV-1. In addition, related sequences are found in the enhancer elements of a number of cellular promoters, including those of the class I MHC, interleukin-2 receptor, and interferon-beta genes. It may act in T-cell activation. Involved in activating HIV-1 gene expression. Isoform 2 and isoform 3 also bind to the IPCS, IRF1 and p53 common sequence DNA sequence in the promoter region of interferon regulatory factor 1 and p53 genes and are involved in transcription regulation of these genes. Isoform 2 does not activate HIV-1 gene expression. Isoform 2 and isoform 3 may be involved in apoptosis.
Subcellular locationIsoform 1: Nucleus.;
SUBCELLULAR LOCATION: Isoform 2: Cytoplasm. Nucleus.;
SUBCELLULAR LOCATION: Isoform 3: Cytoplasm {ECO:0000269|Ref.2}. Nucleus {ECO:0000269|Ref.2}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P15822
Gene Ontology
(Biological Process)
Complete annatation
multicellular organism development [GO:0007275];
signal transduction [GO:0007165];
transcription from RNA polymerase II promoter [GO:0006366]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
metal ion binding [GO:0046872];
RNA polymerase II distal enhancer sequence-specific DNA binding [GO:0000980];
sequence-specific DNA binding [GO:0043565];
transcriptional repressor activity, RNA polymerase II distal enhancer sequence-specific binding [GO:0001206];
transcription regulatory region DNA binding [GO:0044212]
Gene Ontology
(Cellular Component)
Complete annatation
mitochondrion [GO:0005739];
nucleus [GO:0005634]
Protein-protein interaction109343
Phylogenetic treeP15822
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.726485917719923.43699469440573e-072.94331727466381e-06
AZA vs. DISU0.2931655145078590.2491336229414160.831029075563411
AZA vs. IL7-0.114665320304390.5543581969191720.999311006273513
AZA vs. SAHA0.06432987942080690.7928870500137790.945044626544454
DISU vs. CD3-1.446617262059870.0001154591091858670.000643888498656219
DISU vs. IL7-0.4165268159149010.09996141147131630.419349831144291
DISU vs. SAHA-0.2276079590282660.436544533511740.782731980601549
DMSO vs. AZA0.02270152562518130.8933487502565631
DMSO vs. CD3-1.715574781436372.09704294373836e-071.7244515255141e-06
DMSO vs. DISU-0.2723766011355110.2669537187766680.785127350110144
DMSO vs. IL7-0.1301533817138010.4732015375202580.879177319897947
DMSO vs. SAHA0.03449976140563630.884111963056350.971193040903484
HIV vs. Mock in Activation0.3702606132562040.6019547838544520.999983755607037
HIV vs. Mock in Latency-0.0227905497380790.8911474108829770.999834320637052
IL7 vs. CD3-1.833437173956374.27643757072715e-085.69926998263222e-07
SAHA vs. CD3-1.687669734886963.74244797607393e-062.6305359607945e-05
SAHA vs. IL70.1746643555852930.4754048360255870.708758035629482
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.536659 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
204512_at 1.91 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1BBO NMR - A=2087-2143.
3ZNF NMR - A=2114-2143.
4ZNF NMR - A=2114-2143.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat enhanced by 1727488

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found