Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001287
UniProt IDO00291
Primary gene name(s)HIP1
Synonym gene name(s)unknown
Protein nameHuntingtin-interacting protein 1
Protein functionPlays a role in clathrin-mediated endocytosis and trafficking, PubMed:11532990, PubMed:11577110, PubMed:11889126. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner, By similarity. Regulates presynaptic nerve terminal activity, By similarity. Enhances androgen receptor, AR-mediated transcription, PubMed:16027218. May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway, PubMed:11007801. Binds 3-phosphoinositides, via ENTH domain, PubMed:14732715. May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis, PubMed:14732715. May play a functional role in the cell filament networks, PubMed:18790740. May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors, PubMed:11007801, PubMed:12163454. {ECO:0000250|UniProtKB:Q8VD75, ECO:0000269|PubMed:11007801, ECO:0000269|PubMed:11532990, ECO:0000269|PubMed:11577110, ECO:0000269|PubMed:11889126, ECO:0000269|PubMed:12163454, ECO:0000269|PubMed:14732715, ECO:0000269|PubMed:16027218, ECO:0000269|PubMed:18790740, ECO:0000269|PubMed:9147654}.
Subcellular locationCytoplasm. Nucleus. Endomembrane system. Cytoplasmic vesicle, clathrin-coated vesicle membrane. Note=Shuttles between cytoplasm and nucleus. Nuclear translocation can be induced by AR.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O00291
Gene Ontology
(Biological Process)
Complete annatation
activation of cysteine-type endopeptidase activity involved in apoptotic process [GO:0006919];
apoptotic process [GO:0006915];
apoptotic signaling pathway [GO:0097190];
cell differentiation [GO:0030154];
clathrin coat assembly [GO:0048268];
clathrin-dependent endocytosis [GO:0072583];
neurotransmitter receptor transport [GO:0099637];
positive regulation of epidermal growth factor receptor signaling pathway [GO:0045742];
positive regulation of platelet-derived growth factor receptor-beta signaling pathway [GO:2000588];
positive regulation of protein kinase B signaling [GO:0051897];
positive regulation of receptor-mediated endocytosis [GO:0048260];
protein stabilization [GO:0050821];
regulation of apoptotic process [GO:0042981];
regulation of endocytosis [GO:0030100];
regulation of transcription, DNA-templated [GO:0006355];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
clathrin binding [GO:0030276];
phosphatidylinositol binding [GO:0035091];
structural constituent of cytoskeleton [GO:0005200]
Gene Ontology
(Cellular Component)
Complete annatation
clathrin-coated vesicle [GO:0030136];
clathrin-coated vesicle membrane [GO:0030665];
cytoplasm [GO:0005737];
cytoskeleton [GO:0005856];
Golgi apparatus [GO:0005794];
intracellular membrane-bounded organelle [GO:0043231];
membrane [GO:0016020];
nucleus [GO:0005634]
Protein-protein interaction109339
Phylogenetic treeO00291
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.2950895894312080.5982182868899350.703016942634625
AZA vs. DISU0.2355266734683280.6431028904300640.960057478314726
AZA vs. IL7-0.2969288115621060.5623322165573030.999311006273513
AZA vs. SAHA0.445124734403350.3432493933315970.715087497949467
DISU vs. CD30.5183196312865930.317356250242380.449947620391046
DISU vs. IL7-0.5439167052436770.2514609400644670.637747925886918
DISU vs. SAHA0.2150881251286320.6122149617167750.877894725250339
DMSO vs. AZA-0.00658904105511180.9900329744875091
DMSO vs. CD30.28870440258560.6027841956821330.698319921272813
DMSO vs. DISU-0.2410804798167060.6264670226650960.944952148943607
DMSO vs. IL7-0.2844098619074280.5675514169600370.903190909123399
DMSO vs. SAHA0.4461720575987190.3253599939444310.679061136786667
HIV vs. Mock in Activation0.6802324527244210.4114657260803550.999983755607037
HIV vs. Mock in Latency1.212291987736461.25619514790287e-119.01708840927995e-09
IL7 vs. CD3-0.00109063062428560.9983293272970040.998925780028395
SAHA vs. CD30.7188652341135930.1459171871785890.235814692845184
SAHA vs. IL70.742789333136410.08908450952310540.267825326204554
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.59204 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2NO2 X-ray 2.8Å A=482-586.
2QA7 X-ray 2.8Å A/B/C/D=370-481.
3I00 X-ray 2.3Å A/B=361-480.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef requires 25496667

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa05016 Huntington's disease - Homo sapiens (human)