Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001280
UniProt IDQ15011
Primary gene name(s)HERPUD1
Synonym gene name(s)HERP, KIAA0025, MIF1
Protein nameHomocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein
Protein functionComponent of the endoplasmic reticulum quality control, ERQC system also called ER-associated degradation, ERAD involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Could enhance presenilin-mediated beta-amyloid protein 40 generation. Binds to ubiquilins and this interaction is required for efficient degradation of CD3D via the ERAD pathway, PubMed:18307982. {ECO:0000269|PubMed:16289116, ECO:0000269|PubMed:18307982}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:10922362};
Multi-pass membrane protein {ECO:0000269|PubMed:10922362}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q15011
Gene Ontology
(Biological Process)
Complete annatation
endoplasmic reticulum calcium ion homeostasis [GO:0032469];
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [GO:0043154];
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway [GO:1902236];
negative regulation of intrinsic apoptotic signaling pathway [GO:2001243];
PERK-mediated unfolded protein response [GO:0036499];
positive regulation of ER-associated ubiquitin-dependent protein catabolic process [GO:1903071];
positive regulation of protein binding [GO:0032092];
regulation of protein ubiquitination [GO:0031396];
response to endoplasmic reticulum stress [GO:0034976];
response to unfolded protein [GO:0006986];
retrograde protein transport, ER to cytosol [GO:0030970];
ubiquitin-dependent protein catabolic process [GO:0006511]
Gene Ontology
(Molecular Function)
Complete annatation
ion channel binding [GO:0044325]
Gene Ontology
(Cellular Component)
Complete annatation
calcium channel complex [GO:0034704];
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
Lewy body core [GO:1990037];
membrane [GO:0016020]
Protein-protein interaction115060
Phylogenetic treeQ15011
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.405448505858510.2160499953150420.323849381341454
AZA vs. DISU0.416657655533180.100525037928910.641773180085401
AZA vs. IL70.1656916004575580.3892620309632740.999311006273513
AZA vs. SAHA-0.2781563521730010.2549394859816230.626909920340453
DISU vs. CD3-0.001498016122920090.9966993853721180.997559692922071
DISU vs. IL7-0.2601318245565260.3030102589016990.686697148051504
DISU vs. SAHA-0.6925741513717910.01799812818265050.151620899855073
DMSO vs. AZA0.01509827748610830.9283109326952081
DMSO vs. CD3-0.4015051221538190.2096043403304010.310429550440178
DMSO vs. DISU-0.4032173686725660.09933507509115420.568834036745206
DMSO vs. IL70.1578311784212150.3805349207725210.833558583708238
DMSO vs. SAHA-0.2990562718081170.2052987563285470.546100884249781
HIV vs. Mock in Activation-0.1128936513650450.8559078506172530.999983755607037
HIV vs. Mock in Latency-0.1511788324640540.3593513153550780.999834320637052
IL7 vs. CD3-0.2329654801534010.4682876139995960.600521788846845
SAHA vs. CD3-0.7073110622426910.04611618528188720.0927246046962028
SAHA vs. IL7-0.4469245750047610.06717652255710660.22358539463782
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.30312 0.0215429
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1WGD NMR - A=10-90.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)