Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001277
UniProt IDO95714
Primary gene name(s)HERC2
Synonym gene name(s)unknown
Protein nameE3 ubiquitin-protein ligase HERC2
Protein functionE3 ubiquitin-protein ligase that regulates ubiquitin-dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation, IR and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity. {ECO:0000269|PubMed:20023648, ECO:0000269|PubMed:20304803, ECO:0000269|PubMed:22508508}.
Subcellular locationCytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Nucleus. Note=Recruited to sites of DNA damage in response to ionizing radiation, IR via its interaction with RNF8. May loose association with centrosomes during mitosis.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O95714
Gene Ontology
(Biological Process)
Complete annatation
cellular response to DNA damage stimulus [GO:0006974];
double-strand break repair via nonhomologous end joining [GO:0006303];
intracellular protein transport [GO:0006886];
protein sumoylation [GO:0016925];
protein ubiquitination [GO:0016567];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
spermatogenesis [GO:0007283]
Gene Ontology
(Molecular Function)
Complete annatation
guanyl-nucleotide exchange factor activity [GO:0005085];
ligase activity [GO:0016874];
SUMO binding [GO:0032183];
ubiquitin protein ligase binding [GO:0031625];
ubiquitin-protein transferase activity [GO:0004842];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
centriole [GO:0005814];
cytoplasm [GO:0005737];
membrane [GO:0016020];
mitochondrial inner membrane [GO:0005743];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction114438
Phylogenetic treeO95714
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.243619611492450.0001816958031503060.000814792961940318
AZA vs. DISU-0.00446335681911470.9859199983683420.998932793566471
AZA vs. IL7-0.07534740270585060.6946839564663060.999311006273513
AZA vs. SAHA0.4095551973521760.09328450700308490.38461998319598
DISU vs. CD31.226256705652670.0008431151781393980.00357600399416806
DISU vs. IL7-0.08025594899228380.750079815948070.939560944033259
DISU vs. SAHA0.4162563893098170.1546132694702960.506229977012128
DMSO vs. AZA0.1469749018674880.3795584301131611
DMSO vs. CD31.37893900933982.24619449449293e-050.000119109187115579
DMSO vs. DISU0.1495459178471750.539571590971960.921635859189505
DMSO vs. IL7-0.2149879255409770.2313377849472210.740062385273267
DMSO vs. SAHA0.2566844613287230.2763092563878410.624662820771499
HIV vs. Mock in Activation0.3236958455552270.60439829511020.999983755607037
HIV vs. Mock in Latency0.1625617719042270.3236487854299930.999834320637052
IL7 vs. CD31.174416641679140.0003072424849445140.00154060744363562
SAHA vs. CD31.629270496889046.98360563666789e-064.59977871261298e-05
SAHA vs. IL70.4816503786433550.04817731899109010.179236378077438
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.914597 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.067 1.127 1.189 0.984 0.972
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2KEO NMR - A=1203-1296.
3KCI X-ray 1.8Å A=3951-4321.
4L1M X-ray 2.6Å A/B/C=417-790.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)