Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001276
UniProt IDQ15751
Primary gene name(s)HERC1
Synonym gene name(s)unknown
Protein nameProbable E3 ubiquitin-protein ligase HERC1
Protein functionInvolved in membrane trafficking via some guanine nucleotide exchange factor, GEF activity and its ability to bind clathrin. Acts as a GEF for Arf and Rab, by exchanging bound GDP for free GTP. Binds phosphatidylinositol 4,5-bisphosphate, which is required for GEF activity. May also act as a E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. {ECO:0000269|PubMed:15642342, ECO:0000269|PubMed:8861955, ECO:0000269|PubMed:9233772}.
Subcellular locationMembrane;
Peripheral membrane protein. Cytoplasm, cytosol. Golgi apparatus. Note=Recruited onto actin-rich surface protrusions.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q15751
Gene Ontology
(Biological Process)
Complete annatation
cerebellar Purkinje cell differentiation [GO:0021702];
negative regulation of autophagy [GO:0010507];
neuromuscular process controlling balance [GO:0050885];
neuron projection development [GO:0031175];
transport [GO:0006810]
Gene Ontology
(Molecular Function)
Complete annatation
ARF guanyl-nucleotide exchange factor activity [GO:0005086];
ligase activity [GO:0016874];
ubiquitin-protein transferase activity [GO:0004842]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
Golgi apparatus [GO:0005794];
membrane [GO:0016020]
Protein-protein interaction114439
Phylogenetic treeQ15751
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.112852345370966.46811715299123e-109.69060255764252e-09
AZA vs. DISU0.09162998806920770.7171095526778250.973233942294547
AZA vs. IL7-0.2320794503160730.2260665467601050.989265982093417
AZA vs. SAHA0.1031213942577030.6717950869313760.903966067364274
DISU vs. CD32.192197557294776.71188804535205e-091.08907427767101e-07
DISU vs. IL7-0.3330945908192670.1861979589086980.559386040161558
DISU vs. SAHA0.0129004537274040.9647620454870740.992637214624418
DMSO vs. AZA0.08068862132234150.6285815050239131
DMSO vs. CD32.18316089290516.666345253592e-111.08220356945435e-09
DMSO vs. DISU-0.01252654776870490.9590091486430240.994105021699463
DMSO vs. IL7-0.3056539586763960.08831250519348420.554481889126104
DMSO vs. SAHA0.0153903905764490.9478479929874260.987880038071194
HIV vs. Mock in Activation0.3687925499538960.5574053132770640.999983755607037
HIV vs. Mock in Latency0.1969586363690320.2313583880148610.999834320637052
IL7 vs. CD31.887469375888621.38880422717591e-082.03237340934199e-07
SAHA vs. CD32.191067916157713.48082807022365e-095.13422876001324e-08
SAHA vs. IL70.3312049319258890.1735065058258420.398998790056254
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0267885 0.931343
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.036 1.05 1.147 1.231 1.107
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4O2W X-ray 2.0Å A/B/C/D=3975-4360.
4QT6 X-ray 1.6Å A=2035-2192.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)