Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001254
UniProt IDP51610
Primary gene name(s)HCFC1
Synonym gene name(s)HCF1, HFC1
Protein nameHost cell factor 1
Protein functionInvolved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase, H3K4me and Sin3 histone deacetylase, HDAC complexes, involved in the activation and repression of transcription, respectively together. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. In case of human herpes simplex virus, HSV infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes. {ECO:0000269|PubMed:10629049, ECO:0000269|PubMed:10675337, ECO:0000269|PubMed:10779346, ECO:0000269|PubMed:10920196, ECO:0000269|PubMed:12244100, ECO:0000269|PubMed:12670868, ECO:0000269|PubMed:14532282, ECO:0000269|PubMed:15190068, ECO:0000269|PubMed:16624878, ECO:0000269|PubMed:17578910, ECO:0000269|PubMed:20018852, ECO:0000269|PubMed:20200153, ECO:0000269|PubMed:9990006}.
Subcellular locationCytoplasm. Nucleus. Note=HCFC1R1 modulates its subcellular localization and overexpression of HCFC1R1 leads to accumulation of HCFC1 in the cytoplasm. Nuclear in general, but uniquely cytoplasmic in trigeminal ganglia, becoming nuclear upon HSV reactivation from the latent state. Non-processed HCFC1 associates with chromatin. Colocalizes with CREB3 and CANX in the ER.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P51610
Gene Ontology
(Biological Process)
Complete annatation
cell cycle [GO:0007049];
cellular response to organic cyclic compound [GO:0071407];
histone H4-K16 acetylation [GO:0043984];
histone H4-K5 acetylation [GO:0043981];
histone H4-K8 acetylation [GO:0043982];
mitochondrion organization [GO:0007005];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
positive regulation of cell cycle [GO:0045787];
positive regulation of gene expression [GO:0010628];
protein stabilization [GO:0050821];
regulation of protein complex assembly [GO:0043254];
regulation of transcription, DNA-templated [GO:0006355];
release from viral latency [GO:0019046];
transcription from RNA polymerase II promoter [GO:0006366]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
identical protein binding [GO:0042802];
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding [GO:0001205];
transcription coactivator activity [GO:0003713];
transcription factor activity, sequence-specific DNA binding [GO:0003700]
Gene Ontology
(Cellular Component)
Complete annatation
axon [GO:0030424];
cytoplasm [GO:0005737];
dendrite [GO:0030425];
histone acetyltransferase complex [GO:0000123];
membrane [GO:0016020];
mitochondrion [GO:0005739];
MLL1 complex [GO:0071339];
MLL5-L complex [GO:0070688];
neuronal cell body [GO:0043025];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
Set1C/COMPASS complex [GO:0048188]
Protein-protein interaction109304
Phylogenetic treeP51610
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.6273334809662870.05603924323842220.109905461820364
AZA vs. DISU-0.0408875349748290.8714463233243920.991631204917382
AZA vs. IL70.1963636677814490.3074589573342990.999311006273513
AZA vs. SAHA-0.9331845902294930.000215533041946880.00675456356404839
DISU vs. CD3-0.6807712211040840.06112243712625260.1269468011536
DISU vs. IL70.2281098507225840.3653674768151480.74250670167004
DISU vs. SAHA-0.889912000654720.002671015096407770.0417581623630306
DMSO vs. AZA0.06268453055712230.7081274019426251
DMSO vs. CD3-0.5762287798873510.07200306208119540.130344404019852
DMSO vs. DISU0.1016079756365770.6767960749830890.955350023416557
DMSO vs. IL70.1412772915820360.4317007313170040.858560491372538
DMSO vs. SAHA-1.001543996376925.42387462109106e-050.00220927606525577
HIV vs. Mock in Activation0.434597711447830.4853734162851590.999983755607037
HIV vs. Mock in Latency-0.04947883670571640.7643918944448450.999834320637052
IL7 vs. CD3-0.4249533172643330.1861552563418650.305489186591283
SAHA vs. CD3-1.58418402082411.3154567175877e-057.96141876869345e-05
SAHA vs. IL7-1.132817115368683.28160705915792e-050.000792218382335653
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.160177 0.269219
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.048 0.953 0.927 0.897 1.023
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4GO6 X-ray 2.7Å A/C=360-402# B/D=1806-2035.
4N39 X-ray 1.7Å B=1082-1097.
4N3A X-ray 1.8Å B=1072-1097.
4N3B X-ray 2.1Å B=1072-1097.
4N3C X-ray 2.5Å B=1072-1097.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat downregulates 25472883

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa05168 Herpes simplex infection - Homo sapiens (human)