Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001230
UniProt IDP32780
Primary gene name(s)GTF2H1
Synonym gene name(s)BTF2
Protein nameGeneral transcription factor IIH subunit 1
Protein functionComponent of the core-TFIIH basal transcription factor involved in nucleotide excision repair, NER of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P32780
Gene Ontology
(Biological Process)
Complete annatation
7-methylguanosine mRNA capping [GO:0006370];
DNA repair [GO:0006281];
global genome nucleotide-excision repair [GO:0070911];
nucleotide-excision repair, DNA duplex unwinding [GO:0000717];
nucleotide-excision repair, DNA incision [GO:0033683];
nucleotide-excision repair, DNA incision, 3'-to lesion [GO:0006295];
nucleotide-excision repair, DNA incision, 5'-to lesion [GO:0006296];
nucleotide-excision repair, preincision complex assembly [GO:0006294];
nucleotide-excision repair, preincision complex stabilization [GO:0006293];
phosphorylation of RNA polymerase II C-terminal domain [GO:0070816];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
regulation of cyclin-dependent protein serine/threonine kinase activity [GO:0000079];
termination of RNA polymerase I transcription [GO:0006363];
transcription-coupled nucleotide-excision repair [GO:0006283];
transcription elongation from RNA polymerase II promoter [GO:0006368];
transcription elongation from RNA polymerase I promoter [GO:0006362];
transcription from RNA polymerase II promoter [GO:0006366];
transcription from RNA polymerase I promoter [GO:0006360];
transcription initiation from RNA polymerase II promoter [GO:0006367];
transcription initiation from RNA polymerase I promoter [GO:0006361]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682]
Gene Ontology
(Cellular Component)
Complete annatation
core TFIIH complex [GO:0000439];
holo TFIIH complex [GO:0005675];
nucleoplasm [GO:0005654]
Protein-protein interaction109220
Phylogenetic treeP32780
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is not observed
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.029572559003290.001854811797398550.00621238298008687
AZA vs. DISU0.378062450055790.136136382257170.70713984360599
AZA vs. IL70.1475289867036620.4450781878951360.999311006273513
AZA vs. SAHA-0.2517488185869330.3039799366499580.675471598238813
DISU vs. CD3-0.6646050053184060.06780731164252920.137827321426576
DISU vs. IL7-0.2394774748878140.3428147812340610.723800115840821
DISU vs. SAHA-0.6286806068805540.03176463406501220.213907417334137
DMSO vs. AZA-0.05742411178723860.7336532028395221
DMSO vs. CD3-1.097973960966120.0006865543041220110.0024602617962888
DMSO vs. DISU-0.4372752085531650.07407809703645050.507930210315633
DMSO vs. IL70.2120984328119660.2406830200127120.745473115343036
DMSO vs. SAHA-0.2014871887597670.3944882025685520.738825860682323
HIV vs. Mock in Activation-0.1818491856335660.7699972550352450.999983755607037
HIV vs. Mock in Latency0.008867978083551410.9573855138068780.999834320637052
IL7 vs. CD3-0.873666721188990.006909108324860470.021966038764105
SAHA vs. CD3-1.306336610845290.0002759121683880710.00116830468603771
SAHA vs. IL7-0.4028278303464020.09971803000592370.28664179716043
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0627084 0.730617
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.915 1.015 1.106 1.013 1.001
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
202451_at 1.85 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1PFJ NMR - A=1-108.
2DII NMR - A=103-150.
2RNR NMR - B=1-108.
2RUK NMR - B=1-108.
2RVB NMR - B=1-108.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat synergizes with 8934526
23986795
Vpr enhanced by 12379213
21489275
Tat stimulates 8934526
9184228
9311822
9334327
9570510
9651670
10438593
10866664
14569024
Vpr inhibited by 12379213
21489275
Tat binds 8628270
8934526
9184228
9334327
9765201
HIV-1 virus replication enhanced by expression of human gene 18976975
Tat requires 8934526
9184228
9334327
Tat interacts with 8849451
8934526
9054383
91214299651670
10066804
10082552
10866664
14569024

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03022 Basal transcription factors - Homo sapiens (human)
hsa03420 Nucleotide excision repair - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)
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