Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001229
UniProt IDP52655
Primary gene name(s)GTF2A1
Synonym gene name(s)TF2A1
Protein nameTranscription initiation factor IIA subunit 1
Protein functionTFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. {ECO:0000269|PubMed:11030333, ECO:0000269|PubMed:16537915}.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P52655
Gene Ontology
(Biological Process)
Complete annatation
regulation of transcription, DNA-templated [GO:0006355];
snRNA transcription from RNA polymerase II promoter [GO:0042795];
transcription elongation from RNA polymerase II promoter [GO:0006368];
transcription from RNA polymerase II promoter [GO:0006366];
transcription initiation from RNA polymerase II promoter [GO:0006367]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
protein heterodimerization activity [GO:0046982];
TBP-class protein binding [GO:0017025];
transcription coactivator activity [GO:0003713];
transcription factor binding [GO:0008134]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nucleoplasm [GO:0005654];
transcription factor TFIIA complex [GO:0005672]
Protein-protein interaction109212
Phylogenetic treeP52655
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is also observed
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9221500592403690.005248554974023540.0150994885605045
AZA vs. DISU-0.07372490419825430.7715550426997650.976444104025318
AZA vs. IL70.0613689953134740.750589156864010.999311006273513
AZA vs. SAHA-0.1217005019070520.6187259308903640.87646258614782
DISU vs. CD3-1.008663648034620.006120072558331690.0192315976119016
DISU vs. IL70.1262327020812730.6183804727574480.884532273158604
DISU vs. SAHA-0.0473786956485640.8712596251000070.966103245960785
DMSO vs. AZA-0.138301997721440.4121109051119151
DMSO vs. CD3-1.070624838754860.0009410899270193560.00324651242284099
DMSO vs. DISU-0.06615819182612610.7869568185791140.973311777316403
DMSO vs. IL70.20663049333790.253111757953220.75678576796551
DMSO vs. SAHA0.008612065616551590.9709313650039470.993292390076406
HIV vs. Mock in Activation-0.02559812245117810.9671948761141980.999983755607037
HIV vs. Mock in Latency0.02737270425234130.8688146924804830.999834320637052
IL7 vs. CD3-0.8518603483209290.008424360843683030.0258932281804311
SAHA vs. CD3-1.069601766459350.002762329320259660.00855141800663786
SAHA vs. IL7-0.1874805474260340.4426056402685060.68268059156937
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.804856 0.00108419
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.98 1.054 1.14 1.265 1.399
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1NVP X-ray 2.1Å B=2-58# C=303-376.
5FUR EM 8.5Å B=9-51# C=330-376.
5IY6 EM 7.2Å N=1-376.
5IY7 EM 8.6Å N=1-376.
5IY8 EM 7.9Å N=1-376.
5IY9 EM 6.3Å N=1-376.
5IYA EM 5.4Å N=1-376.
5IYB EM 3.9Å N=1-376.
5IYC EM 3.9Å N=1-376.
5IYD EM 3.9Å N=1-376.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat stabilizes 8764062
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03022 Basal transcription factors - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)