Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001223
UniProt IDQ9NRY4
Primary gene name(s)ARHGAP35
Synonym gene name(s)GRF1, GRLF1, KIAA1722, P190A, p190ARHOGAP
Protein nameRho GTPase-activating protein 35
Protein functionRho GTPase-activating protein, GAP, binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity, PubMed:19673492. This binding is inhibited by phosphorylation by PRKCA, PubMed:19673492. Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis, By similarity. Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein, GAP, By similarity. Transduces SRC-dependent signal from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation, By similarity. Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation, By similarity. During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth, By similarity. Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences, PubMed:1894621. {ECO:0000250|UniProtKB:P81128, ECO:0000250|UniProtKB:Q91YM2, ECO:0000269|PubMed:1894621, ECO:0000269|PubMed:19673492}.
Subcellular locationCytoplasm {ECO:0000250|UniProtKB:Q91YM2}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q91YM2}. Cell membrane {ECO:0000250|UniProtKB:Q91YM2}. Nucleus {ECO:0000305|PubMed:1894621}. Note=In response to integrins and SDC4 and upon phosphorylation by PKC, relocalizes from the cytoplasm to regions of plasma membrane ruffling where it colocalizes with polymerized actin. {ECO:0000250|UniProtKB:Q91YM2}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NRY4
Gene Ontology
(Biological Process)
Complete annatation
axonal fasciculation [GO:0007413];
axon guidance [GO:0007411];
camera-type eye development [GO:0043010];
cell migration [GO:0016477];
cellular response to extracellular stimulus [GO:0031668];
central nervous system neuron axonogenesis [GO:0021955];
establishment or maintenance of actin cytoskeleton polarity [GO:0030950];
forebrain development [GO:0030900];
mammary gland development [GO:0030879];
negative regulation of Rho protein signal transduction [GO:0035024];
negative regulation of transcription, DNA-templated [GO:0045892];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
negative regulation of vascular permeability [GO:0043116];
neural tube closure [GO:0001843];
neuron projection guidance [GO:0097485];
positive regulation of cilium assembly [GO:0045724];
positive regulation of neuron projection development [GO:0010976];
regulation of actin cytoskeleton organization [GO:0032956];
regulation of actin polymerization or depolymerization [GO:0008064];
regulation of axonogenesis [GO:0050770];
regulation of cell shape [GO:0008360];
regulation of small GTPase mediated signal transduction [GO:0051056];
small GTPase mediated signal transduction [GO:0007264];
transcription, DNA-templated [GO:0006351];
wound healing, spreading of cells [GO:0044319]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
GTPase activator activity [GO:0005096];
GTP binding [GO:0005525];
RNA polymerase II regulatory region sequence-specific DNA binding [GO:0000977];
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding [GO:0001227];
transcription corepressor activity [GO:0003714]
Gene Ontology
(Cellular Component)
Complete annatation
actin cytoskeleton [GO:0015629];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleus [GO:0005634]
Protein-protein interaction109166
Phylogenetic treeQ9NRY4
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.7217163874317690.02831654157986150.0620600428517773
AZA vs. DISU0.0569669260909160.8218123324156450.984767732537381
AZA vs. IL7-0.1246518464144350.5174697617489910.999311006273513
AZA vs. SAHA0.2066369246214020.3980397082512260.758362757462495
DISU vs. CD30.7663385357633280.03544388700327870.0820078515253144
DISU vs. IL7-0.1911690127240190.4481089132497060.79531291736317
DISU vs. SAHA0.1518583543218870.6026857082800870.87408232064678
DMSO vs. AZA0.02699433046443040.8722777872679811
DMSO vs. CD30.7392627777857890.02144343398323490.0474787379420097
DMSO vs. DISU-0.0314089451813680.8975823899764090.988283279918411
DMSO vs. IL7-0.1445091652185150.422402577215920.854003087820276
DMSO vs. SAHA0.173130965104390.4642747978323450.792317765830935
HIV vs. Mock in Activation0.3958178659478750.526251322346960.999983755607037
HIV vs. Mock in Latency0.2132842360931310.1972238147391860.999834320637052
IL7 vs. CD30.603062360797970.06137356405050240.129564672173432
SAHA vs. CD30.9043967104607430.01129597220995360.0284641179985558
SAHA vs. IL70.328366827277050.1791974652946940.406871907192681
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.244537 0.0695029
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.022 1.063 1.209 1.369 1.201
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2K85 NMR - A=267-331.
3C5H X-ray 1.8Å A=13-249.
3FK2 X-ray 2.8Å A/B/C/D=1212-1439.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope transmembrane glycoprotein gp41 activates 20617170

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04510 Focal adhesion - Homo sapiens (human)
hsa04611 Platelet activation - Homo sapiens (human)
hsa04670 Leukocyte transendothelial migration - Homo sapiens (human)
hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human)