Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001197
UniProt IDP00505
Primary gene name(s)GOT2
Synonym gene name(s)unknown
Protein nameAspartate aminotransferase, mitochondrial
Protein functionCatalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid, KA. Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. {ECO:0000269|PubMed:9537447}.
Subcellular locationMitochondrion matrix {ECO:0000269|PubMed:9537447}. Cell membrane {ECO:0000269|PubMed:9537447}. Note=Exposure to alcohol promotes translocation to the cell membrane.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P00505
Gene Ontology
(Biological Process)
Complete annatation
2-oxoglutarate metabolic process [GO:0006103];
4-hydroxyproline catabolic process [GO:0019470];
aspartate biosynthetic process [GO:0006532];
aspartate catabolic process [GO:0006533];
aspartate metabolic process [GO:0006531];
cellular amino acid biosynthetic process [GO:0008652];
fatty acid transport [GO:0015908];
gluconeogenesis [GO:0006094];
glutamate catabolic process to 2-oxoglutarate [GO:0019551];
glutamate catabolic process to aspartate [GO:0019550];
glutamate metabolic process [GO:0006536];
oxaloacetate metabolic process [GO:0006107];
response to ethanol [GO:0045471]
Gene Ontology
(Molecular Function)
Complete annatation
amino acid binding [GO:0016597];
identical protein binding [GO:0042802];
kynurenine-oxoglutarate transaminase activity [GO:0016212];
L-aspartate:2-oxoglutarate aminotransferase activity [GO:0004069];
L-phenylalanine:2-oxoglutarate aminotransferase activity [GO:0080130];
phospholipid binding [GO:0005543];
poly(A RNA binding [GO:0044822];
pyridoxal phosphate binding [GO:0030170]
Gene Ontology
(Cellular Component)
Complete annatation
cell surface [GO:0009986];
extracellular exosome [GO:0070062];
mitochondrial inner membrane [GO:0005743];
mitochondrial matrix [GO:0005759];
mitochondrion [GO:0005739];
myelin sheath [GO:0043209];
perikaryon [GO:0043204];
plasma membrane [GO:0005886];
protein complex [GO:0043234]
Protein-protein interaction109068
Phylogenetic treeP00505
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.297014923957410.0001010969963748390.000487750937695765
AZA vs. DISU-0.04417975903597910.8617591480284380.991133096247651
AZA vs. IL70.2346810735087890.2250866018053870.989265982093417
AZA vs. SAHA0.2651287152915420.2789748713276410.652186946198663
DISU vs. CD3-1.352939350387790.0002467711498265190.00124385632905324
DISU vs. IL70.269474823815960.2860691473703190.672985704838881
DISU vs. SAHA0.3110048725745960.2873198697656820.669883061289726
DMSO vs. AZA-0.01488501016687550.9298274302876831
DMSO vs. CD3-1.323957414878864.83092581620159e-050.000234918971662611
DMSO vs. DISU0.02722221016316050.9114114963146970.988283279918411
DMSO vs. IL70.2569950667201180.1555606239044690.660212951959422
DMSO vs. SAHA0.2743448934750440.2463804025623210.594656797374496
HIV vs. Mock in Activation0.07003009145142690.9103165482658450.999983755607037
HIV vs. Mock in Latency-0.05873765733625830.7246178963371150.999834320637052
IL7 vs. CD3-1.055555635201670.001158006817465580.00481906039747619
SAHA vs. CD3-1.055763420542740.003247398529814480.00982696703700889
SAHA vs. IL70.02804632321986550.9086317603751220.965237149816391
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0858154 0.610916
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.037 0.957 1.026 1.024 0.835
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00128 L-Aspartic Acid approved, nutraceutical unknown unknown
DB02783 4&,39;-Deoxy-4&,39;-Acetylyamino-Pyridoxal-5&,39;-Phosphate experimental unknown unknown
DB00114 Pyridoxal Phosphate nutraceutical unknown cofactor
DB00142 L-Glutamic Acid nutraceutical unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
5AX8 X-ray 2.9Å A/B/C/D=30-430.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00220 Arginine biosynthesis - Homo sapiens (human)
hsa00250 Alanine# aspartate and glutamate metabolism - Homo sapiens (human)
hsa00270 Cysteine and methionine metabolism - Homo sapiens (human)
hsa00330 Arginine and proline metabolism - Homo sapiens (human)
hsa00350 Tyrosine metabolism - Homo sapiens (human)
hsa00360 Phenylalanine metabolism - Homo sapiens (human)
hsa00400 Phenylalanine# tyrosine and tryptophan biosynthesis - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01200 Carbon metabolism - Homo sapiens (human)
hsa01210 2-Oxocarboxylic acid metabolism - Homo sapiens (human)
hsa01230 Biosynthesis of amino acids - Homo sapiens (human)
hsa04975 Fat digestion and absorption - Homo sapiens (human)