Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001190
UniProt IDQ9NVN8
Primary gene name(s)GNL3L
Synonym gene name(s)unknown
Protein nameGuanine nucleotide-binding protein-like 3-like protein
Protein functionStabilizes TERF1 telomeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP. {ECO:0000269|PubMed:16251348, ECO:0000269|PubMed:17034816, ECO:0000269|PubMed:19487455, ECO:0000269|PubMed:21132010}.
Subcellular locationNucleus, nucleolus {ECO:0000269|PubMed:17034816}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NVN8
Gene Ontology
(Biological Process)
Complete annatation
negative regulation of protein binding [GO:0032091];
negative regulation of protein sumoylation [GO:0033234];
negative regulation of protein ubiquitination [GO:0031397];
negative regulation of telomere maintenance via telomerase [GO:0032211];
positive regulation of protein homodimerization activity [GO:0090073];
positive regulation of protein localization to chromosome, telomeric region [GO:1904816];
regulation of protein stability [GO:0031647];
ribosome biogenesis [GO:0042254]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase activity [GO:0003924];
GTP binding [GO:0005525];
poly(A RNA binding [GO:0044822]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
membrane [GO:0016020];
mitochondrion [GO:0005739];
nucleolus [GO:0005730];
nucleus [GO:0005634]
Protein-protein interaction120037
Phylogenetic treeQ9NVN8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5127128899608880.1194401795950070.202862563314847
AZA vs. DISU-0.4817597537231610.08841739446382210.617324596640878
AZA vs. IL70.3131436557299310.142036347099230.907697229500996
AZA vs. SAHA-0.502431373327650.04209398513445810.242740907389755
DISU vs. CD3-1.005388185984020.006112118759226790.0192146148439176
DISU vs. IL70.7857831163648410.004929175095767290.0747583195653716
DISU vs. SAHA-0.02070315451951270.9442329669715140.987159724983301
DMSO vs. AZA-0.09780560531465080.566541409728911
DMSO vs. CD3-0.6204037419018610.0542736056285480.103301562702979
DMSO vs. DISU0.3823671550127930.1225172756352390.604917205712392
DMSO vs. IL70.4179572143327580.0219559745490830.302823711187033
DMSO vs. SAHA-0.4134344349458220.08302852143340060.335831193068102
HIV vs. Mock in Activation0.1042615406710980.8671427291201470.999983755607037
HIV vs. Mock in Latency-0.06656651732862340.6938840557897130.999834320637052
IL7 vs. CD3-0.1906189506593380.5537685619353620.676798308120628
SAHA vs. CD3-1.041258309845890.003750723931433940.0111376176643763
SAHA vs. IL7-0.8209502191199790.0009111914074529940.0108493675165454
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.10416 0.514892
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.524 0.422 0.398 0.487 0.566
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03008 Ribosome biogenesis in eukaryotes - Homo sapiens (human)