Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001183
UniProt IDP49915
Primary gene name(s)GMPS
Synonym gene name(s)unknown
Protein nameGMP synthase [glutamine-hydrolyzing]
Protein functionInvolved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division.
Subcellular locationCytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P49915
Gene Ontology
(Biological Process)
Complete annatation
glutamine metabolic process [GO:0006541];
purine nucleobase biosynthetic process [GO:0009113];
purine ribonucleoside monophosphate biosynthetic process [GO:0009168]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
GMP synthase, glutamine-hydrolyzing activity [GO:0003922];
GMP synthase activity [GO:0003921];
pyrophosphatase activity [GO:0016462]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829]
Protein-protein interaction114360
Phylogenetic treeP49915
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.2207065969840.0002498965534686310.0010804477492482
AZA vs. DISU-0.06205582036300640.80777121104740.983470094122325
AZA vs. IL70.2159096247300720.2631172929563990.999311006273513
AZA vs. SAHA0.2133093397971370.3836555917635730.747090090459127
DISU vs. CD3-1.293850282927230.0004377659397830640.00203858009771081
DISU vs. IL70.269272997161770.2937282507874930.677929701118574
DISU vs. SAHA0.275387638934930.3534109929022990.73062511303574
DMSO vs. AZA-0.1727061883548460.3051206570970991
DMSO vs. CD3-1.403078419412151.73787015872451e-059.44488494102399e-05
DMSO vs. DISU-0.1121308808273960.6482396101056980.948871187480749
DMSO vs. IL70.3957085582283740.0285038449711640.344031595628741
DMSO vs. SAHA0.3776861837099050.1111428471671270.395708445465574
HIV vs. Mock in Activation-0.02423244468442850.9688954208250430.999983755607037
HIV vs. Mock in Latency0.02368189273956690.8863228138398820.999834320637052
IL7 vs. CD3-0.9955701418494850.002147999163847230.00819665604978968
SAHA vs. CD3-1.033137971814280.004231891135423550.012366608379064
SAHA vs. IL7-0.006881489024097510.9775985316114150.991743742210813
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.21269 0.158781
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.004 0.936 0.832 0.789 0.781
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00142 L-Glutamic Acid nutraceutical unknown unknown
DB00130 L-Glutamine approved, investigational, nutraceutical unknown substrate

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2VPI X-ray 2.4Å A/B=25-219.
2VXO X-ray 2.5Å A/B=20-693.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00230 Purine metabolism - Homo sapiens (human)
hsa00983 Drug metabolism - other enzymes - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)