Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001175
UniProt IDO94925
Primary gene name(s)GLS
Synonym gene name(s)GLS1, KIAA0838
Protein nameGlutaminase kidney isoform, mitochondrial
Protein functionCatalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
Subcellular locationIsoform 1: Cytoplasm, cytosol.;
SUBCELLULAR LOCATION: Isoform 3: Mitochondrion.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O94925
Gene Ontology
(Biological Process)
Complete annatation
cellular amino acid biosynthetic process [GO:0008652];
chemical synaptic transmission [GO:0007268];
glutamate biosynthetic process [GO:0006537];
glutamate secretion [GO:0014047];
glutamine catabolic process [GO:0006543];
protein homotetramerization [GO:0051289];
regulation of respiratory gaseous exchange by neurological system process [GO:0002087];
suckling behavior [GO:0001967]
Gene Ontology
(Molecular Function)
Complete annatation
glutaminase activity [GO:0004359]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
mitochondrial matrix [GO:0005759];
mitochondrion [GO:0005739]
Protein-protein interaction109006
Phylogenetic treeO94925
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.578911719123882.62038673992659e-061.85333031178666e-05
AZA vs. DISU0.1713854015019150.4973278730500420.934754900374469
AZA vs. IL70.2865550684722820.144345107821550.908692496481083
AZA vs. SAHA0.1270931708790690.6023840989599130.868743947405718
DISU vs. CD3-1.420400498930560.0001319484417970920.000721694779263193
DISU vs. IL70.1067040137864820.6712608246562090.909512068237771
DISU vs. SAHA-0.04384026837099630.8806778052552640.96945856056033
DMSO vs. AZA-0.08840483213580820.5964056461442851
DMSO vs. CD3-1.678348828866113.46046895915997e-072.70113807615259e-06
DMSO vs. DISU-0.2615352437459250.2830725993780530.798111145486858
DMSO vs. IL70.3822332308293250.03320658736774220.37273961620902
DMSO vs. SAHA0.207854205949760.3783290073362610.725836311964274
HIV vs. Mock in Activation-0.2470750820901720.6929821811635520.999983755607037
HIV vs. Mock in Latency-0.05945361337171210.7179311655705950.999834320637052
IL7 vs. CD3-1.283103913894818.89864585676969e-050.000525584988240947
SAHA vs. CD3-1.477545722324654.07427796207394e-050.000217496878811386
SAHA vs. IL7-0.1639373977816530.5213745584808870.743538324932913
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.580005 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.962 1.103 1.164 1.173 1.054
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00142 L-Glutamic Acid nutraceutical unknown unknown
DB00130 L-Glutamine approved, investigational, nutraceutical unknown substrate

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3CZD X-ray 2.4Å A=221-533.
3UNW X-ray 2.5Å A/B/C/D=71-597.
3UO9 X-ray 2.3Å A/B/C/D=71-597.
3VOY X-ray 2.2Å A=221-533.
3VOZ X-ray 2.4Å A=221-533.
3VP0 X-ray 2.4Å A=221-533.
3VP1 X-ray 2.3Å A=221-533.
3VP2 X-ray 2.7Å A=221-533.
3VP3 X-ray 2.7Å A=221-533.
3VP4 X-ray 2.4Å A=221-533.
4O7D X-ray 2.3Å A=221-531.
5D3O X-ray 2.7Å A/B=72-597.
5FI2 X-ray 2.5Å A/B/C/D=72-597.
5FI6 X-ray 2.5Å A/B/C/D=72-597.
5FI7 X-ray 2.5Å A/B/C/D=72-597.
5HL1 X-ray 2.4Å A/B/C/D=72-597.
5I94 X-ray 2.9Å A/B/C/D=72-597.
5JYO X-ray 2.1Å A/B/C/D/E/F/G/H=221-533.
5JYP X-ray 2.7Å A=221-533.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00220 Arginine biosynthesis - Homo sapiens (human)
hsa00250 Alanine# aspartate and glutamate metabolism - Homo sapiens (human)
hsa00471 D-Glutamine and D-glutamate metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04724 Glutamatergic synapse - Homo sapiens (human)
hsa04727 GABAergic synapse - Homo sapiens (human)
hsa04964 Proximal tubule bicarbonate reclamation - Homo sapiens (human)
hsa05206 MicroRNAs in cancer - Homo sapiens (human)
hsa05230 Central carbon metabolism in cancer - Homo sapiens (human)