Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001173
UniProt IDO76003
Primary gene name(s)GLRX3
Synonym gene name(s)PICOT, TXNL2
Protein nameGlutaredoxin-3
Protein functionCritical negative regulator of cardiac hypertrophy and a positive inotropic regulator, By similarity. Crucial regulator of cellular iron homeostasis and hemoglobin maturation. May play a role in regulating the function of the thioredoxin system. Does not posses any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity. {ECO:0000250, ECO:0000269|PubMed:23615448}.
Subcellular locationCytoplasm, cell cortex {ECO:0000269|PubMed:10636891}. Cytoplasm, myofibril, sarcomere, Z line {ECO:0000250}. Note=Under the plasma membrane. After PMA stimulation, GLRX3 and PRKCQ/PKC-theta translocate to a more extended submembrane area. In the Z line, found associated with CSRP3, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O76003
Gene Ontology
(Biological Process)
Complete annatation
cell redox homeostasis [GO:0045454];
negative regulation of cardiac muscle hypertrophy [GO:0010614];
regulation of the force of heart contraction [GO:0002026]
Gene Ontology
(Molecular Function)
Complete annatation
electron carrier activity [GO:0009055];
iron-sulfur cluster binding [GO:0051536];
metal ion binding [GO:0046872];
poly(A RNA binding [GO:0044822];
protein disulfide oxidoreductase activity [GO:0015035]
Gene Ontology
(Cellular Component)
Complete annatation
cell cortex [GO:0005938];
dendrite [GO:0030425];
extracellular exosome [GO:0070062];
nucleus [GO:0005634];
Z disc [GO:0030018]
Protein-protein interaction115793
Phylogenetic treeO76003
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.772705510089451.4363051870081e-071.33464807512861e-06
AZA vs. DISU0.04258205119881770.8667680347720790.991211477030548
AZA vs. IL70.2886053181467750.1357116964808450.899503583997056
AZA vs. SAHA0.0305936344837460.9008559694574440.97791670980362
DISU vs. CD3-1.743790911449862.85646120368188e-062.44527226156721e-05
DISU vs. IL70.2372758527507460.3476039365181360.728706625366555
DISU vs. SAHA-0.01045313544089110.9714464868182180.994982935027721
DMSO vs. AZA-0.1065137319634050.5289143850820671
DMSO vs. CD3-1.891058254643151.02896277054398e-081.10172879447371e-07
DMSO vs. DISU-0.1510081980581970.5372877767953840.92112338311561
DMSO vs. IL70.4022826806589590.0263702604576480.330450271320901
DMSO vs. SAHA0.1304102878803720.5822873459813880.856662689504637
HIV vs. Mock in Activation-0.3877956032117110.5332676753573630.999983755607037
HIV vs. Mock in Latency-0.1055050490171940.5270198676797750.999834320637052
IL7 vs. CD3-1.475485719721596.70426441429228e-065.41841166308995e-05
SAHA vs. CD3-1.767107449601681.20320589236389e-069.55841475905315e-06
SAHA vs. IL7-0.2613281215913340.2860159943681270.533322849047964
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0511753 0.791682
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
0.06 0.004

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.015 1.064 1.192 1.178 1.13
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2DIY NMR - A=1-117.
2WZ9 X-ray 1.5Å A=1-125.
2YAN X-ray 1.9Å A/B=232-334.
3ZYW X-ray 1.8Å A/B=130-232.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found