Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001171
UniProt IDQ9BZE0
Primary gene name(s)GLIS2
Synonym gene name(s)NKL
Protein nameZinc finger protein GLIS2
Protein functionCan act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway, By similarity. Represses the Hedgehog-dependent expression of Wnt4, By similarity. Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition, By similarity. Represses transcriptional activation mediated by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation, By similarity. {ECO:0000250}.
Subcellular locationNucleus speckle {ECO:0000250}. Cytoplasm {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9BZE0
Gene Ontology
(Biological Process)
Complete annatation
cell differentiation involved in kidney development [GO:0061005];
negative regulation of sequence-specific DNA binding transcription factor activity [GO:0043433];
negative regulation of smoothened signaling pathway [GO:0045879];
negative regulation of transcription, DNA-templated [GO:0045892];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
nervous system development [GO:0007399];
positive regulation of protein localization to nucleus [GO:1900182];
positive regulation of transcription, DNA-templated [GO:0045893];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
regulation of transcription from RNA polymerase II promoter involved in kidney development [GO:0060994]
Gene Ontology
(Molecular Function)
Complete annatation
metal ion binding [GO:0046872];
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding [GO:0001077];
transcription regulatory region DNA binding [GO:0044212]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nuclear speck [GO:0016607];
nucleus [GO:0005634]
Protein-protein interaction124182
Phylogenetic treeQ9BZE0
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.198317843750560.002841362504286130.00898501296669008
AZA vs. DISU0.2193501055223990.5618841986618130.951335690540517
AZA vs. IL70.3699325120969160.1559844188148030.922020841096286
AZA vs. SAHA1.064382359676444.9372183564933e-050.00222825238041258
DISU vs. CD3-0.9906434850155930.05971046872758060.124560698256234
DISU vs. IL70.1391243594607290.7322836095300490.933273103880286
DISU vs. SAHA0.8473542330484840.02695538140795710.19383061119217
DMSO vs. AZA0.06767192539771530.7340179632994671
DMSO vs. CD3-1.14565590913150.002382002810076830.00724946915834495
DMSO vs. DISU-0.1551225129400850.6614624431019130.951258614137401
DMSO vs. IL70.3113156912633650.1644928582932390.671831438301428
DMSO vs. SAHA0.9934279942115378.05576777557215e-050.00298506449707957
HIV vs. Mock in Activation0.3407580226810370.6624941943424870.999983755607037
HIV vs. Mock in Latency0.2236144140652250.2798498296507180.999834320637052
IL7 vs. CD3-0.8280344028050890.05408194955508880.117241079482792
SAHA vs. CD3-0.1579146472541060.6877836403516320.770338124124793
SAHA vs. IL70.6930293973108380.01103367564658790.066661974627922
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0596184 0.898123
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found