Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001144
UniProt IDQ8IWJ2
Primary gene name(s)GCC2
Synonym gene name(s)KIAA0336, RANBP2L4
Protein nameGRIP and coiled-coil domain-containing protein 2
Protein functionGolgin which probably tethers transport vesicles to the trans-Golgi network, TGN and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector it is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. May also play a role in transport between the recycling endosomes and the Golgi. Required for maintenance of the Golgi structure, it is involved in the biogenesis of noncentrosomal, Golgi-associated microtubules through recruitment of CLASP1 and CLASP2. {ECO:0000269|PubMed:16885419, ECO:0000269|PubMed:17488291, ECO:0000269|PubMed:17543864}.
Subcellular locationCytoplasm. Golgi apparatus, trans-Golgi network membrane;
Peripheral membrane protein.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8IWJ2
Gene Ontology
(Biological Process)
Complete annatation
Golgi ribbon formation [GO:0090161];
late endosome to Golgi transport [GO:0034499];
microtubule anchoring [GO:0034453];
microtubule organizing center organization [GO:0031023];
protein localization to Golgi apparatus [GO:0034067];
protein targeting to Golgi [GO:0000042];
protein targeting to lysosome [GO:0006622];
recycling endosome to Golgi transport [GO:0071955];
regulation of protein exit from endoplasmic reticulum [GO:0070861];
retrograde transport, endosome to Golgi [GO:0042147]
Gene Ontology
(Molecular Function)
Complete annatation
identical protein binding [GO:0042802]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
Golgi apparatus [GO:0005794];
membrane [GO:0016020];
trans-Golgi network [GO:0005802]
Protein-protein interaction115006
Phylogenetic treeQ8IWJ2
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.382510906620950.2428191932200650.354285661201629
AZA vs. DISU0.2372015131201930.3496265353801810.887429206006287
AZA vs. IL7-0.1321109142047740.4916188958862430.999311006273513
AZA vs. SAHA0.2230757803234880.3600748558267440.7285422903643
DISU vs. CD30.6064385880128070.09680407950521630.181744263851243
DISU vs. IL7-0.3777641323211220.1342843492195350.478950468966487
DISU vs. SAHA-0.01388686660995880.9621510563227540.992306752196005
DMSO vs. AZA-0.1087074698114270.5157812344962351
DMSO vs. CD30.2620917490204160.4121807459416020.526362883581438
DMSO vs. DISU-0.347736083602190.1554328250510050.657838022105253
DMSO vs. IL7-0.01624742212666320.9279357614614830.985392477038029
DMSO vs. SAHA0.3238977760121250.1693697917827740.497625345667461
HIV vs. Mock in Activation0.006946397553568310.991139681257170.999983755607037
HIV vs. Mock in Latency0.05850361443412060.7223608444837110.999834320637052
IL7 vs. CD30.2597212967028250.4190996871853580.555903589556705
SAHA vs. CD30.5793125041703320.1016522440347930.176204824822483
SAHA vs. IL70.3502592132517610.1508479294806770.367942020872448
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.106705 0.508811
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.973 1.055 1.022 1.062 0.944
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3BBP X-ray 3.0Å D/E/F=1547-1612.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found