Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001133
UniProt IDQ14697
Primary gene name(s)GANAB
Synonym gene name(s)G2AN, KIAA0088
Protein nameNeutral alpha-glucosidase AB
Protein functionCleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2Man(9GlcNAc(2 oligosaccharide precursor of immature glycoproteins, PubMed:10929008. Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia, PubMed:27259053. {ECO:0000269|PubMed:10929008, ECO:0000269|PubMed:27259053}.
Subcellular locationEndoplasmic reticulum {ECO:0000250}. Golgi apparatus {ECO:0000250}. Melanosome {ECO:0000269|PubMed:10929008, ECO:0000269|PubMed:17081065}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q14697
Gene Ontology
(Biological Process)
Complete annatation
carbohydrate metabolic process [GO:0005975];
protein folding [GO:0006457]
Gene Ontology
(Molecular Function)
Complete annatation
carbohydrate binding [GO:0030246];
glucan 1,3-alpha-glucosidase activity [GO:0033919];
poly(A RNA binding [GO:0044822]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum lumen [GO:0005788];
extracellular exosome [GO:0070062];
glucosidase II complex [GO:0017177];
Golgi apparatus [GO:0005794];
melanosome [GO:0042470];
membrane [GO:0016020]
Protein-protein interaction116802
Phylogenetic treeQ14697
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.292111390186370.0001325909783372880.000620732859005554
AZA vs. DISU-0.2030590142182620.4211089533608550.912170766340211
AZA vs. IL7-0.06703913901665250.7263872415825590.999311006273513
AZA vs. SAHA-0.09824576093661210.6864198550873860.909717060895527
DISU vs. CD31.077117727688230.003853499407254660.0130623955771018
DISU vs. IL70.1266040158434680.6144430627253630.882584961465922
DISU vs. SAHA0.1068443373470550.7133119656878180.915550590566598
DMSO vs. AZA0.07372696457976960.6584493632854711
DMSO vs. CD31.353165276706844.10515076242346e-050.000203300776580899
DMSO vs. DISU0.2745152769786770.2597081386864360.778711032631234
DMSO vs. IL7-0.1332332384596710.4571135950457330.872512684612464
DMSO vs. SAHA-0.1773452421915690.4510388695587340.781963281159517
HIV vs. Mock in Activation-0.04098131878630390.9572136755668040.999983755607037
HIV vs. Mock in Latency-0.01778854641298260.9137611149571710.999834320637052
IL7 vs. CD31.231699315180170.0001958835737911890.00104700464813993
SAHA vs. CD31.170397757777890.001361075894797280.0046669883355165
SAHA vs. IL7-0.0340218434451630.8886432911523120.956187928924358
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0912866 0.580292
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.038 0.921 0.988 1.105 0.944
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00491 Miglitol approved yes antagonist

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 processed by 12560567
1704656
29598668416962
8794361
8794362
11752220
Envelope surface glycoprotein gp160; precursor interacts with 22190034
Envelope surface glycoprotein gp120 interacts with 22190034
Envelope transmembrane glycoprotein gp41 processed by 1704656
1736542
2829950
3099781
3264072
8093218
Envelope surface glycoprotein gp120 glycosylated by 2076345
2136376
2283726
2355006
2649653
2959866
8218172
12719582
16547752
Envelope surface glycoprotein gp160; precursor processed by 1678778
28251772187500
2541446
2542563
2649653
2829950
8673525
9109416
11530211
HIV-1 virus replication enhanced by expression of human gene 18854154

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00510 N-Glycan biosynthesis - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)
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