Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001128
UniProt IDO75293
Primary gene name(s)GADD45B
Synonym gene name(s)MYD118
Protein nameGrowth arrest and DNA damage-inducible protein GADD45 beta
Protein functionInvolved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O75293
Gene Ontology
(Biological Process)
Complete annatation
activation of MAPKK activity [GO:0000186];
activation of MAPKKK activity [GO:0000185];
apoptotic process [GO:0006915];
cell differentiation [GO:0030154];
multicellular organism development [GO:0007275];
negative regulation of protein kinase activity [GO:0006469];
positive regulation of apoptotic process [GO:0043065];
positive regulation of JNK cascade [GO:0046330];
positive regulation of p38MAPK cascade [GO:1900745];
regulation of cell cycle [GO:0051726];
response to stress [GO:0006950]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nucleus [GO:0005634]
Protein-protein interaction110701
Phylogenetic treeO75293
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.758846977814011.03375797300131e-067.98605851823466e-06
AZA vs. DISU-0.125696334223520.6208344512279510.960057478314726
AZA vs. IL70.1056715060974310.5852389003815410.999311006273513
AZA vs. SAHA0.1763275591859830.4724546788542330.805931508933019
DISU vs. CD3-2.897520404804785.94404414688832e-076.06638601693937e-06
DISU vs. IL70.2220012217340450.3803630355602490.751208274737424
DISU vs. SAHA0.3039178891899090.2983968723570470.682418704596821
DMSO vs. AZA-0.07025751311837110.6782653117389871
DMSO vs. CD3-2.842787484682585.19771257811996e-073.93090060187637e-06
DMSO vs. DISU0.05291804470452790.8290777363482930.980987097956162
DMSO vs. IL70.1835582805679410.3111088261605510.793157559033593
DMSO vs. SAHA0.2410152069575190.3097503984265370.661221186848131
HIV vs. Mock in Activation0.6276860920018090.5510323730152850.999983755607037
HIV vs. Mock in Latency-0.2992848205837620.07738729297746590.999834320637052
IL7 vs. CD3-2.64687011003122.47830503974367e-062.20525258362639e-05
SAHA vs. CD3-2.607080712181684.08144774044494e-062.85020470104288e-05
SAHA vs. IL70.06767520927017850.7823230351375710.904628523447663
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.526205 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
209304_x_at 1.42 No downregulated in CD8+ cells
207574_s_at 1.55 No downregulated in CD8+ cells
209305_s_at 1.52 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04010 MAPK signaling pathway - Homo sapiens (human)
hsa04064 NF-kappa B signaling pathway - Homo sapiens (human)
hsa04068 FoxO signaling pathway - Homo sapiens (human)
hsa04110 Cell cycle - Homo sapiens (human)
hsa04115 p53 signaling pathway - Homo sapiens (human)
hsa04210 Apoptosis - Homo sapiens (human)