Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001125
UniProt IDQ9UBS5
Primary gene name(s)GABBR1
Synonym gene name(s)GPRC3A
Protein nameGamma-aminobutyric acid type B receptor subunit 1
Protein functionComponent of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins, G proteins and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Calcium is required for high affinity binding to GABA. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium, Kir conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by, --baclofen, cgp27492 and blocked by phaclofen.; FUNCTION: Isoform 1E may regulate the formation of functional GABBR1/GABBR2 heterodimers by competing for GABBR2 binding. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.
Subcellular locationCell membrane;
Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane;
Multi-pass membrane protein. Cell projection, dendrite {ECO:0000250}. Note=Colocalizes with ATF4 in hippocampal neuron dendritic membranes, By similarity. Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. {ECO:0000250}.;
SUBCELLULAR LOCATION: Isoform 1E: Secreted {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UBS5
Gene Ontology
(Biological Process)
Complete annatation
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway [GO:0007193];
gamma-aminobutyric acid signaling pathway [GO:0007214];
negative regulation of adenylate cyclase activity [GO:0007194]
Gene Ontology
(Molecular Function)
Complete annatation
G-protein coupled GABA receptor activity [GO:0004965]
Gene Ontology
(Cellular Component)
Complete annatation
axolemma [GO:0030673];
cell junction [GO:0030054];
dendritic shaft [GO:0043198];
dendritic spine [GO:0043197];
endoplasmic reticulum membrane [GO:0005789];
extracellular space [GO:0005615];
G-protein coupled receptor heterodimeric complex [GO:0038039];
integral component of plasma membrane [GO:0005887];
membrane raft [GO:0045121];
mitochondrial membrane [GO:0031966];
neuronal cell body [GO:0043025];
plasma membrane [GO:0005886];
postsynaptic membrane [GO:0045211];
presynaptic membrane [GO:0042734];
synaptic vesicle [GO:0008021]
Protein-protein interaction108825
Phylogenetic treeQ9UBS5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.4307458568955330.1970854159427780.301845408068349
AZA vs. DISU-0.1215953313354490.7232867707498550.974208899704183
AZA vs. IL7-0.4940910408201480.2570517350796840.999311006273513
AZA vs. SAHA1.292565748809961.82358112633674e-050.00105320543672031
DISU vs. CD30.2941708074862390.4212976199554470.553818812523625
DISU vs. IL7-0.3808201770878070.346234150319230.726969669842606
DISU vs. SAHA1.415523362369731.91767590784764e-060.000168030776785882
DMSO vs. AZA0.2065121422687530.6046651568523851
DMSO vs. CD30.6182172859732350.0581126830915770.109296825315182
DMSO vs. DISU0.3243845564093980.3620805969108370.847989928915865
DMSO vs. IL7-0.6923643254875690.1231524812140720.617606006088536
DMSO vs. SAHA1.081199144682720.0005957131469985780.0123324077450763
HIV vs. Mock in Activation0.1231328606516450.8438150688786630.999983755607037
HIV vs. Mock in Latency-0.3140916161087070.05906748580287540.950248965840494
IL7 vs. CD3-0.05513187039509840.8803325498607110.924042654878168
SAHA vs. CD31.698509513813693.13495210846337e-062.26154754784792e-05
SAHA vs. IL71.781487035732632.92776698962438e-060.000114039172097152
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 2.1351 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00181 Baclofen approved yes agonist
DB00837 Progabide approved, investigational yes agonist
DB05010 SGS742 investigational unknown unknown
DB01080 Vigabatrin approved yes agonist
DB08891 Arbaclofen investigational yes agonist
DB08892 Arbaclofen Placarbil investigational yes agonist

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4MQE X-ray 2.3Å A=165-576.
4MQF X-ray 2.2Å A=165-576.
4MR7 X-ray 2.1Å A=165-576.
4MR8 X-ray 2.1Å A=165-576.
4MR9 X-ray 2.3Å A=165-576.
4MRM X-ray 2.8Å A=165-576.
4MS1 X-ray 2.2Å A=165-576.
4MS3 X-ray 2.5Å A=165-576.
4MS4 X-ray 1.9Å A=165-576.
4PAS X-ray 1.6Å A=879-919.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04024 cAMP signaling pathway - Homo sapiens (human)
hsa04080 Neuroactive ligand-receptor interaction - Homo sapiens (human)
hsa04727 GABAergic synapse - Homo sapiens (human)
hsa04742 Taste transduction - Homo sapiens (human)
hsa04915 Estrogen signaling pathway - Homo sapiens (human)
hsa05032 Morphine addiction - Homo sapiens (human)