Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001124
UniProt IDQ9H0R8
Primary gene name(s)GABARAPL1
Synonym gene name(s)GEC1
Protein nameGamma-aminobutyric acid receptor-associated protein-like 1
Protein functionUbiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. {ECO:0000269|PubMed:16431922, ECO:0000269|PubMed:20404487}.
Subcellular locationCytoplasm, cytoskeleton. Cytoplasmic vesicle membrane;
Lipid-anchor. Endoplasmic reticulum {ECO:0000250}. Golgi apparatus {ECO:0000250}. Cytoplasmic vesicle, autophagosome.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9H0R8
Gene Ontology
(Biological Process)
Complete annatation
autophagosome assembly [GO:0000045];
cellular response to nitrogen starvation [GO:0006995];
macroautophagy [GO:0016236];
mitophagy [GO:0000422]
Gene Ontology
(Molecular Function)
Complete annatation
beta-tubulin binding [GO:0048487];
GABA receptor binding [GO:0050811];
Tat protein binding [GO:0030957];
ubiquitin protein ligase binding [GO:0031625]
Gene Ontology
(Cellular Component)
Complete annatation
autophagosome [GO:0005776];
autophagosome membrane [GO:0000421];
cell body [GO:0044297];
cytoplasmic vesicle membrane [GO:0030659];
cytosol [GO:0005829];
dendrite cytoplasm [GO:0032839];
dendrite membrane [GO:0032590];
endoplasmic reticulum [GO:0005783];
Golgi apparatus [GO:0005794];
intracellular [GO:0005622];
microtubule [GO:0005874]
Protein-protein interaction117223
Phylogenetic treeQ9H0R8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9377213655429050.004702206470281860.0137775380871089
AZA vs. DISU1.483412399718463.60704588759919e-050.00765811404361552
AZA vs. IL7-0.01240825553345230.9490968917013670.999311006273513
AZA vs. SAHA1.036260815663073.42959757352324e-050.00169500832207506
DISU vs. CD30.5309124742523130.1561564105367140.2640383322973
DISU vs. IL7-1.50470965379442.23037305728813e-050.00164014018724968
DISU vs. SAHA-0.4449058655503780.2663194697362750.644124516135771
DMSO vs. AZA-0.0753590799436580.6576493355694181
DMSO vs. CD3-1.024531600598090.001550817439058270.00499851620013112
DMSO vs. DISU-1.560666579025578.47298463946267e-060.00205948191155972
DMSO vs. IL70.06999664596775530.7001828945785210.937788846060647
DMSO vs. SAHA1.104583086427984.55560365408836e-060.000297218626280371
HIV vs. Mock in Activation-0.3571364777066230.5666313849319770.999983755607037
HIV vs. Mock in Latency-0.2215504562950.1830414930110430.999834320637052
IL7 vs. CD3-0.9418398601512690.003718910540569160.0130878212883491
SAHA vs. CD30.07344128992257030.835650119179640.884158248247949
SAHA vs. IL71.045310097193132.58118208593672e-050.000667461255778458
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.882022 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
208868_s_at 1.6 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2L8J NMR - A=2-115.
2R2Q X-ray 1.6Å A/B=3-111.
5DPT X-ray 2.9Å A/B=2-117.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04068 FoxO signaling pathway - Homo sapiens (human)
hsa04140 Autophagy - Homo sapiens (human)
hsa04621 NOD-like receptor signaling pathway - Homo sapiens (human)
hsa04727 GABAergic synapse - Homo sapiens (human)