Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001123
UniProt IDO95166
Primary gene name(s)GABARAP
Synonym gene name(s)FLC3B
Protein nameGamma-aminobutyric acid receptor-associated protein
Protein functionUbiquitin-like modifier that plays a role in intracellular transport of GABA(A receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. {ECO:0000269|PubMed:15977068}.
Subcellular locationEndomembrane system {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P60517}. Golgi apparatus membrane {ECO:0000250}. Cytoplasmic vesicle, autophagosome {ECO:0000269|PubMed:12507496, ECO:0000269|PubMed:15169837, ECO:0000269|PubMed:17580304, ECO:0000269|PubMed:19056683}. Cytoplasmic vesicle {ECO:0000250|UniProtKB:P60517}. Note=Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules, By similarity. Localizes also to discrete punctae along the ciliary axoneme, By similarity. {ECO:0000250|UniProtKB:P60517, ECO:0000250|UniProtKB:Q9DCD6}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O95166
Gene Ontology
(Biological Process)
Complete annatation
chemical synaptic transmission [GO:0007268];
extrinsic apoptotic signaling pathway via death domain receptors [GO:0008625];
macroautophagy [GO:0016236];
microtubule cytoskeleton organization [GO:0000226];
protein targeting [GO:0006605]
Gene Ontology
(Molecular Function)
Complete annatation
beta-tubulin binding [GO:0048487];
GABA receptor binding [GO:0050811];
ubiquitin protein ligase binding [GO:0031625]
Gene Ontology
(Cellular Component)
Complete annatation
actin cytoskeleton [GO:0015629];
autophagosome [GO:0005776];
autophagosome membrane [GO:0000421];
axoneme [GO:0005930];
cell body [GO:0044297];
cytoplasmic vesicle [GO:0031410];
cytosol [GO:0005829];
Golgi membrane [GO:0000139];
lysosome [GO:0005764];
microtubule [GO:0005874];
microtubule associated complex [GO:0005875];
perinuclear region of cytoplasm [GO:0048471];
plasma membrane [GO:0005886];
smooth endoplasmic reticulum [GO:0005790];
sperm midpiece [GO:0097225]
Protein-protein interaction116465
Phylogenetic treeO95166
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is not observed
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.9281195770983190.005549687167045620.0158208596523003
AZA vs. DISU0.1976424082931830.4347239075183560.915763738642415
AZA vs. IL7-0.2882901272557660.1348823592119130.898662602392564
AZA vs. SAHA0.3226793091493960.1881466236578550.553605739804509
DISU vs. CD31.113176710861150.002713440282589460.00972940029014119
DISU vs. IL7-0.4956956395220920.04967378576872610.293890235660732
DISU vs. SAHA0.1273858386879620.6630773617576170.897300791869358
DMSO vs. AZA-0.04549576541522640.7864732763159661
DMSO vs. CD30.870944530666030.007676050852226780.0198284818396427
DMSO vs. DISU-0.2451198688328470.3153031652361850.820207449196067
DMSO vs. IL7-0.2355404503123450.191507205529320.701710769886993
DMSO vs. SAHA0.3627014390700980.1258002294277040.423704000443315
HIV vs. Mock in Activation-0.1158965648148040.8690876646951540.999983755607037
HIV vs. Mock in Latency0.1112693080353140.501218364396830.999834320637052
IL7 vs. CD30.6460757628096070.04776357344448620.105687073183902
SAHA vs. CD31.227253861378030.0008917666486627510.00324454934701778
SAHA vs. IL70.6085588530055140.01326352507886420.0763108309308392
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.882022 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.909 1.087 0.955 0.895 1.03
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1GNU X-ray 1.7Å A=1-117.
1KLV NMR - A=1-117.
1KM7 NMR - A=1-117.
1KOT NMR - A=1-117.
3D32 X-ray 1.3Å A/B=1-117.
3DOW X-ray 2.3Å A=1-117.
3WIM X-ray 2.6Å A=1-117.
4XC2 X-ray 1.9Å A/B/C/D=3-116.
5DPS X-ray 2.0Å A/B/C=2-117.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef downregulates 25847297
HIV-1 virus replication enhanced by expression of human gene 18976975
Tat interacts with 24198379

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04068 FoxO signaling pathway - Homo sapiens (human)
hsa04140 Autophagy - Homo sapiens (human)
hsa04621 NOD-like receptor signaling pathway - Homo sapiens (human)
hsa04727 GABAergic synapse - Homo sapiens (human)