Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001119
UniProt IDQ13283
Primary gene name(s)G3BP1
Synonym gene name(s)G3BP
Protein nameRas GTPase-activating protein-binding protein 1
Protein functionMay be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. {ECO:0000269|PubMed:11604510, ECO:0000269|PubMed:9889278}.
Subcellular locationCytoplasm. Cytoplasm, cytosol. Cytoplasmic granule. Cell membrane. Nucleus. Note=Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells, By similarity. Cytosolic and partially nuclear in resting cells. Recruited to stress granules, SGs upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13283
Gene Ontology
(Biological Process)
Complete annatation
negative regulation of canonical Wnt signaling pathway [GO:0090090];
Ras protein signal transduction [GO:0007265];
transport [GO:0006810]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
ATP-dependent DNA helicase activity [GO:0004003];
ATP-dependent RNA helicase activity [GO:0004004];
DNA binding [GO:0003677];
endonuclease activity [GO:0004519];
mRNA binding [GO:0003729];
poly(A RNA binding [GO:0044822]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytoplasmic stress granule [GO:0010494];
cytosol [GO:0005829];
focal adhesion [GO:0005925];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction115448
Phylogenetic treeQ13283
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.123699858941260.000697313397562960.00264666520475168
AZA vs. DISU0.1317020945196970.6024207718482370.955158276076142
AZA vs. IL70.3761065136337980.05006243182208770.655026939977715
AZA vs. SAHA-0.2378594144847550.3287851453183770.700410864970211
DISU vs. CD3-1.004367837417060.006325391506988740.0197655372256112
DISU vs. IL70.2355649398926390.3495260147395420.730805086296615
DISU vs. SAHA-0.3687498696311260.2060592625906160.579387900467011
DMSO vs. AZA-0.04545468761520820.7854532913922151
DMSO vs. CD3-1.179056127937430.000275710790319850.00110379565670171
DMSO vs. DISU-0.1785869517585450.463918816973460.894136919272291
DMSO vs. IL70.4286874345561030.01697902879340770.271466805222618
DMSO vs. SAHA-0.1999090468889660.3956531161850950.739628890352961
HIV vs. Mock in Activation0.02312289607553070.9703723671814470.999983755607037
HIV vs. Mock in Latency0.06680441033295770.6847240549528060.999834320637052
IL7 vs. CD3-0.739389366045040.02211652734323220.0567232289679325
SAHA vs. CD3-1.386614745973780.000116217898720850.000549373818608282
SAHA vs. IL7-0.617646591997020.011324779868090.0679847597497366
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.032083775
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.237779 0.078566
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.961 1.006 0.801 0.643 0.935
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
201503_at 1.48 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3Q90 X-ray 1.7Å A/B=1-139.
4FCJ X-ray 1.6Å A/B=1-139.
4FCM X-ray 2.6Å A/B=1-139.
4IIA X-ray 3.3Å A=11-139.
5FW5 X-ray 1.9Å A/B=1-139.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) binds 25229650
Envelope surface glycoprotein gp120 interacts with 22190034
Pr55(Gag) co-localizes with 25229650
Pr55(Gag) interacts with 25352557
Tat interacts with 25496916

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found