Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001087
UniProt IDP02751
Primary gene name(s)FN1
Synonym gene name(s)FN
Protein nameFibronectin
Protein functionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.; FUNCTION: Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
Subcellular locationSecreted, extracellular space, extracellular matrix.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P02751
Gene Ontology
(Biological Process)
Complete annatation
acute-phase response [GO:0006953];
angiogenesis [GO:0001525];
calcium-independent cell-matrix adhesion [GO:0007161];
cell adhesion [GO:0007155];
cell-substrate junction assembly [GO:0007044];
endodermal cell differentiation [GO:0035987];
extracellular matrix disassembly [GO:0022617];
extracellular matrix organization [GO:0030198];
integrin activation [GO:0033622];
leukocyte migration [GO:0050900];
negative regulation of transforming growth factor-beta secretion [GO:2001202];
peptide cross-linking [GO:0018149];
platelet degranulation [GO:0002576];
positive regulation of axon extension [GO:0045773];
positive regulation of cell proliferation [GO:0008284];
positive regulation of fibroblast proliferation [GO:0048146];
positive regulation of gene expression [GO:0010628];
positive regulation of substrate-dependent cell migration, cell attachment to substrate [GO:1904237];
regulation of cell shape [GO:0008360];
regulation of ERK1 and ERK2 cascade [GO:0070372];
regulation of protein phosphorylation [GO:0001932];
response to wounding [GO:0009611];
substrate adhesion-dependent cell spreading [GO:0034446];
wound healing [GO:0042060]
Gene Ontology
(Molecular Function)
Complete annatation
collagen binding [GO:0005518];
heparin binding [GO:0008201];
identical protein binding [GO:0042802];
integrin binding [GO:0005178];
mercury ion binding [GO:0045340];
peptidase activator activity [GO:0016504];
protease binding [GO:0002020]
Gene Ontology
(Cellular Component)
Complete annatation
apical plasma membrane [GO:0016324];
basal lamina [GO:0005605];
blood microparticle [GO:0072562];
endoplasmic reticulum-Golgi intermediate compartment [GO:0005793];
extracellular exosome [GO:0070062];
extracellular matrix [GO:0031012];
extracellular region [GO:0005576];
extracellular space [GO:0005615];
fibrinogen complex [GO:0005577];
platelet alpha granule lumen [GO:0031093]
Protein-protein interaction108621
Phylogenetic treeP02751
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.2760881880930920.6699886744098640.761164010292796
AZA vs. DISU-0.01571926452954390.9680481106352350.997285288974472
AZA vs. IL7-0.221955548521430.5874012967160140.999311006273513
AZA vs. SAHA0.08895441003301170.82357896730240.956664048171101
DISU vs. CD30.2491679777978960.7063409975226890.79423278854001
DISU vs. IL7-0.2172844672978020.6070849145036330.879473869512038
DISU vs. SAHA0.1071547981947520.7951744875598480.945838966293503
DMSO vs. AZA0.09671532320321770.758194707494251
DMSO vs. CD30.3624056680543250.5498180375253670.653956136548643
DMSO vs. DISU0.1110345909566660.7394431002798680.968014021576247
DMSO vs. IL7-0.3113872157049320.3733514305435790.831458117840584
DMSO vs. SAHA-0.01250711190589650.9704795466886780.993292390076406
HIV vs. Mock in Activation1.240013943829150.2011702970241210.999983755607037
HIV vs. Mock in Latency0.6338286161148740.1872714377127920.999834320637052
IL7 vs. CD30.05603571094467750.9307721318500310.957132746030328
SAHA vs. CD30.3418665533421370.5962518087292880.694344699602069
SAHA vs. IL70.3096760949957870.4701529731054360.704411680408642
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.758827 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB08888 Ocriplasmin approved yes cleavage

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1E88 NMR - A=305-464.
1E8B NMR - A=305-464.
1FBR NMR - A=183-275.
1FNA X-ray 1.8Å A=1452-1542.
1FNF X-ray 2.0Å A=1173-1540.
1FNH X-ray 2.8Å A=1721-1991.
1J8K NMR - A=1631-1724.
1O9A NMR - A=48-140.
1OWW NMR - A=608-701.
1Q38 NMR - A=631-705.
1QGB NMR - A=48-140.
1QO6 NMR - A=305-405.
1TTF NMR - A=1447-1540.
1TTG NMR - A=1447-1540.
2CG6 X-ray 1.5Å A=93-182.
2CG7 X-ray 1.2Å A=93-182.
2CK2 X-ray 2.0Å A/B=1447-1542.
2CKU NMR - A=93-182.
2EC3 NMR - A=2239-2299.
2FN2 NMR - A=406-464.
2FNB NMR - A=1265-1355.
2GEE X-ray 2.0Å A=1205-1356.
2H41 NMR - A=721-809.
2H45 NMR - A=721-809.
2HA1 NMR - A=609-809.
2N1K NMR - A=808-905.
2OCF X-ray 2.9Å D=1447-1540.
2RKY X-ray 1.8Å A/C=183-275.
2RKZ X-ray 2.0Å A/B/C/D/E/F=93-182.
2RL0 X-ray 2.0Å A/B/D/F/I/K=184-272.
3CAL X-ray 1.7Å A/C=93-182.
3EJH X-ray 2.1Å A/B=516-608.
3GXE X-ray 2.6Å A/B=516-608.
3M7P X-ray 2.5Å A=297-604.
3MQL X-ray 3.0Å A=308-515.
3R8Q X-ray 2.4Å A=1721-1991.
3T1W X-ray 2.4Å A=1173-1448.
3ZRZ X-ray 1.7Å A/B=93-182.
4GH7 X-ray 2.6Å B/D=1173-1427.
4JE4 X-ray 2.3Å B=1450-1540.
4JEG X-ray 2.3Å B=1450-1540.
4LXO X-ray 1.4Å A/B=1357-1540.
4MMX X-ray 3.3Å C=1448-1540.
4MMY X-ray 3.1Å C=1448-1540.
4MMZ X-ray 3.1Å C=1448-1540.
4PZ5 X-ray 1.9Å A=93-182.
5DC0 X-ray 2.2Å A=1447-1540.
5DC4 X-ray 1.4Å B=1445-1540.
5DC9 X-ray 1.5Å B=1445-1540.
5DFT X-ray 2.5Å A/B/C/D/E/F/G/H/I/J=1539-1635.
5J7C X-ray 2.5Å C/D=1447-1540.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope transmembrane glycoprotein gp41 binds 8173552
Envelope surface glycoprotein gp160; precursor binds 1875953
Tat competes with 7690138
retropepsin cleaves 1959621
Tat upregulates 1409674
Envelope surface glycoprotein gp120 binds 1875953
Nef upregulates 10451539
Tat modulated by 9339851
Envelope surface glycoprotein gp120 induces phosphorylation of 22241990

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa04510 Focal adhesion - Homo sapiens (human)
hsa04512 ECM-receptor interaction - Homo sapiens (human)
hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human)
hsa04933 AGE-RAGE signaling pathway in diabetic complications - Homo sapiens (human)
hsa05100 Bacterial invasion of epithelial cells - Homo sapiens (human)
hsa05146 Amoebiasis - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05205 Proteoglycans in cancer - Homo sapiens (human)
hsa05222 Small cell lung cancer - Homo sapiens (human)