Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001074
UniProt IDQ96AC1
Primary gene name(s)FERMT2
Synonym gene name(s)KIND2, MIG2, PLEKHC1
Protein nameFermitin family homolog 2
Protein functionScaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling. {ECO:0000269|PubMed:12679033, ECO:0000269|PubMed:18458155, ECO:0000269|PubMed:21325030, ECO:0000269|PubMed:22030399, ECO:0000269|PubMed:22078565, ECO:0000269|PubMed:22699938}.
Subcellular locationCytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Membrane;
Peripheral membrane protein;
Cytoplasmic side. Cell projection, lamellipodium membrane;
Peripheral membrane protein;
Cytoplasmic side. Nucleus. Cytoplasm, myofibril, sarcomere, I band {ECO:0000250}. Cell surface {ECO:0000250}. Note=Colocalizes with actin stress fibers at cell-ECM focal adhesion sites. Colocalizes with ITGB3 at lamellipodia at the leading edge of spreading cells. Binds to membranes that contain phosphatidylinositides.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96AC1
Gene Ontology
(Biological Process)
Complete annatation
cell junction assembly [GO:0034329];
cell-matrix adhesion [GO:0007160];
focal adhesion assembly [GO:0048041];
integrin activation [GO:0033622];
integrin-mediated signaling pathway [GO:0007229];
protein localization to membrane [GO:0072657];
regulation of cell shape [GO:0008360];
substrate adhesion-dependent cell spreading [GO:0034446];
transforming growth factor beta receptor signaling pathway [GO:0007179];
Wnt signaling pathway [GO:0016055]
Gene Ontology
(Molecular Function)
Complete annatation
phosphatidylinositol-3,4,5-trisphosphate binding [GO:0005547]
Gene Ontology
(Cellular Component)
Complete annatation
cell cortex [GO:0005938];
cell surface [GO:0009986];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extrinsic component of cytoplasmic side of plasma membrane [GO:0031234];
filamentous actin [GO:0031941];
focal adhesion [GO:0005925];
I band [GO:0031674];
lamellipodium membrane [GO:0031258];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
stress fiber [GO:0001725]
Protein-protein interaction116175
Phylogenetic treeQ96AC1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3320710724301610.3910360739216780.512450717863449
AZA vs. DISU0.2495007101625030.6677831605741990.962557690454146
AZA vs. IL7-0.3828113129183930.3953339012024370.999311006273513
AZA vs. SAHA0.09525018706346790.8437340127645480.962862177940253
DISU vs. CD3-0.101356648501530.8302605347192760.884726940503207
DISU vs. IL7-0.6393723594247250.2232235083053520.605341677946246
DISU vs. SAHA-0.1531783498893750.7813669684002310.940086709960701
DMSO vs. AZA-0.01719766293121180.9715231832967951
DMSO vs. CD3-0.3688954259815060.2763644229481310.384896924444263
DMSO vs. DISU-0.2708274718492870.6201170841041190.941879588741789
DMSO vs. IL7-0.3570008749604230.3749087019817090.831519022414553
DMSO vs. SAHA0.1061878055687060.8086874102443010.946766717920598
HIV vs. Mock in Activation-0.3743841137196920.5615150285453420.999983755607037
HIV vs. Mock in Latency-0.4504041099099650.3143621831029010.999834320637052
IL7 vs. CD3-0.7089443852525420.03881814158422980.0893837225938124
SAHA vs. CD3-0.2674469393492750.4704204518342480.581848014538913
SAHA vs. IL70.4721076271574140.2446957471787430.487110771217302
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
NOTEST 0.183636 1
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.036 1.215 1.664 2.291 1.761
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2LGX NMR - A=1-105.
2LKO NMR - A=367-500.
2MSU NMR - A=339-358.
4F7H X-ray 1.9Å A=328-499.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found