Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001069
UniProt IDQ0JRZ9
Primary gene name(s)FCHO2
Synonym gene name(s)unknown
Protein nameF-BAR domain only protein 2
Protein functionFunctions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a lipid-binding activity with a preference for membranes enriched in phosphatidylserine and phosphoinositides, Pi(4,5 biphosphate like the plasma membrane. Its membrane-bending activity might be important for the subsequent action of clathrin and adaptors in the formation of clathrin-coated vesicles. Involved in adaptor protein complex AP-2-dependent endocytosis of the transferin receptor, it also functions in the AP-2-independent endocytosis of the LDL receptor. {ECO:0000269|PubMed:17540576, ECO:0000269|PubMed:20448150, ECO:0000269|PubMed:21762413, ECO:0000269|PubMed:22323290}.
Subcellular locationMembrane, clathrin-coated pit {ECO:0000250};
Peripheral membrane protein {ECO:0000250};
Cytoplasmic side {ECO:0000250}. Note=Associated with forming but not mature clathrin-coated vesicles. The recruitment to coated-pits precede the one of clathrin and the adaptor protein complex AP-2, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q0JRZ9
Gene Ontology
(Biological Process)
Complete annatation
clathrin coat assembly [GO:0048268];
clathrin-dependent endocytosis [GO:0072583];
membrane invagination [GO:0010324];
protein localization to plasma membrane [GO:0072659]
Gene Ontology
(Molecular Function)
Complete annatation
phosphatidylinositol-4,5-bisphosphate binding [GO:0005546];
phosphatidylinositol binding [GO:0035091];
phosphatidylserine binding [GO:0001786]
Gene Ontology
(Cellular Component)
Complete annatation
clathrin-coated vesicle [GO:0030136];
coated pit [GO:0005905];
plasma membrane [GO:0005886]
Protein-protein interaction125437
Phylogenetic treeQ0JRZ9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.06435288923193570.8610750054581740.907624482989412
AZA vs. DISU0.7065116170746350.06575309263302840.561305683207042
AZA vs. IL7-0.5366856879147320.09025009765035590.815062745286887
AZA vs. SAHA0.5318363933603920.2019782371928970.569726786572928
DISU vs. CD30.6292897974225780.1001279149506980.186605365938937
DISU vs. IL7-1.248370329627770.0002259821247959650.00973337294656905
DISU vs. SAHA-0.1767060382251970.6778677905203790.903243467560864
DMSO vs. AZA-0.3491549267788410.2524288818926571
DMSO vs. CD3-0.4289913967974570.2227566537383450.325618958719985
DMSO vs. DISU-1.058409421342580.001177776283717850.0693212680122814
DMSO vs. IL7-0.1839655179597840.4580157923600990.872998531019969
DMSO vs. SAHA0.8720974721650920.01682800524195850.12668075274803
HIV vs. Mock in Activation-0.662081018358530.3234213381619870.999983755607037
HIV vs. Mock in Latency-0.144176844427080.4912683291697630.999834320637052
IL7 vs. CD3-0.5860534586227470.09987600291001720.189744264162174
SAHA vs. CD30.4397651960728030.287641950217390.400877817835128
SAHA vs. IL71.055340694888080.004639742582051910.0365983671804555
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.944364 0.00241278
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2V0O X-ray 2.3Å A/B/C=3-274.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found