Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001066
UniProt IDQ9UKT4
Primary gene name(s)FBXO5
Synonym gene name(s)EMI1, FBX5
Protein nameF-box only protein 5
Protein functionRegulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome, APC. Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding. {ECO:0000269|PubMed:11988738, ECO:0000269|PubMed:16921029}.
Subcellular locationNucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Note=In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UKT4
Gene Ontology
(Biological Process)
Complete annatation
cell division [GO:0051301];
G1/S transition of mitotic cell cycle [GO:0000082];
microtubule polymerization [GO:0046785];
mitotic nuclear division [GO:0007067];
negative regulation of meiotic nuclear division [GO:0045835];
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051436];
oocyte maturation [GO:0001556];
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition [GO:0051437];
regulation of mitotic cell cycle [GO:0007346];
regulation of mitotic nuclear division [GO:0007088];
regulation of transcription involved in G1/S transition of mitotic cell cycle [GO:0000083];
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051439];
spindle assembly involved in female meiosis I [GO:0007057];
vesicle organization [GO:0016050]
Gene Ontology
(Molecular Function)
Complete annatation
metal ion binding [GO:0046872];
protein kinase binding [GO:0019901]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
spindle [GO:0005819]
Protein-protein interaction117655
Phylogenetic treeQ9UKT4
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.8391613621953070.01776777286289490.0422457599920416
AZA vs. DISU0.1936483723351020.4497150757655760.918749813367893
AZA vs. IL70.1324392472757080.5507599611808540.999311006273513
AZA vs. SAHA-0.4882962219071070.06684430947800350.318159495128331
DISU vs. CD3-0.658009877597130.07262829563731140.145114041062724
DISU vs. IL7-0.06959800963742350.7846385705776350.950685295889555
DISU vs. SAHA-0.6822267319613260.02094526623672910.167462125970156
DMSO vs. AZA-0.09521982926366250.5821341197737211
DMSO vs. CD3-0.9478808377725860.003954898843859360.0112380265407632
DMSO vs. DISU-0.2913615636841280.2393955615333710.756203026523891
DMSO vs. IL70.2346324360655830.2026656005917230.713365252999855
DMSO vs. SAHA-0.4007439924206480.09665186684875280.365714357338075
HIV vs. Mock in Activation-0.2566552651334520.7289190488368030.999983755607037
HIV vs. Mock in Latency-0.07502662327962460.6579252252497180.999834320637052
IL7 vs. CD3-0.6963041848441010.0535759933319160.116311529236925
SAHA vs. CD3-1.354755383903840.0004755081129087510.00187491032483906
SAHA vs. IL7-0.6260768957237730.01868650679074950.0960710789098763
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.8 0.011468551 1.2 0.16329637 1.2 0.391328588
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.365651 0.00493672
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
0.04 3.08E-04

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
218875_s_at 1.53 Yes upregulated in CD4+ cells
218875_s_at 2.22 Yes upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2M6N NMR - A=364-447.
4UI9 EM 3.6Å S=1-447# U=1-27.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04114 Oocyte meiosis - Homo sapiens (human)