Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001064
UniProt IDQ9UK99
Primary gene name(s)FBXO3
Synonym gene name(s)FBX3
Protein nameF-box only protein 3
Protein functionSubstrate recognition component of the SCF, SKP1-CUL1-F-box protein-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. {ECO:0000269|PubMed:18809579}.
Subcellular locationNucleus. Note=Colocalizes with PML at the peripheries of nuclear bodies.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UK99
Gene Ontology
(Biological Process)
Complete annatation
protein polyubiquitination [GO:0000209];
proteolysis [GO:0006508]
Gene Ontology
(Molecular Function)
Complete annatation
ubiquitin-protein transferase activity [GO:0004842]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleoplasm [GO:0005654]
Protein-protein interaction117657
Phylogenetic treeQ9UK99
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.8518967414635150.009933663866273260.0259345542685728
AZA vs. DISU0.04368486835282670.863061740437330.991133096247651
AZA vs. IL7-0.1746911661359890.3655039729146930.999311006273513
AZA vs. SAHA0.2847592591560230.2449544275960340.616685811960318
DISU vs. CD30.8827521460042630.01568271193473370.0421278872330428
DISU vs. IL7-0.2272371097294720.3678460219365850.743235327797081
DISU vs. SAHA0.2417659638519160.408708751485140.765248644640402
DMSO vs. AZA-0.02841054299334650.8659890217699911
DMSO vs. CD30.8125788596512950.01177308711828530.0286621779956315
DMSO vs. DISU-0.07375444492359740.7627877276553770.969857060964769
DMSO vs. IL7-0.1391817810002640.4408942606555580.863964770490385
DMSO vs. SAHA0.3057016235144620.1962943720695310.534142588952085
HIV vs. Mock in Activation0.05898211860446240.9246799060540950.999983755607037
HIV vs. Mock in Latency0.08957307858163450.5884619437150530.999834320637052
IL7 vs. CD30.6855830620077350.03391763790838010.0803458362711006
SAHA vs. CD31.111550271825310.002007662954921270.00648563359714118
SAHA vs. IL70.454984958778060.06332337341851750.214647297258991
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.7 0.000130817 1.4 0.003674079 1.4 0.008669278
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.862081 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.161 1.182 1.167 0.998 0.946
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
5HDW X-ray 2.0Å A=278-407.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found