Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001057
UniProt IDP22087
Primary gene name(s)FBL
Synonym gene name(s)FIB1, FLRN
Protein namerRNA 2'-O-methyltransferase fibrillarin
Protein functionS-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A, H2AQ104me, a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus, PubMed:24352239. {ECO:0000269|PubMed:24352239}.
Subcellular locationNucleus, nucleolus {ECO:0000269|PubMed:14583623, ECO:0000269|PubMed:2026646, ECO:0000269|PubMed:2414294, ECO:0000269|PubMed:24352239}. Note=Fibrillar region of the nucleolus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P22087
Gene Ontology
(Biological Process)
Complete annatation
box C/D snoRNA 3'-end processing [GO:0000494];
histone glutamine methylation [GO:1990258];
osteoblast differentiation [GO:0001649];
rRNA methylation [GO:0031167];
rRNA processing [GO:0006364];
snoRNA localization [GO:0048254];
tRNA processing [GO:0008033]
Gene Ontology
(Molecular Function)
Complete annatation
ATPase binding [GO:0051117];
histone-glutamine methyltransferase activity [GO:1990259];
poly(A RNA binding [GO:0044822];
RNA binding [GO:0003723];
rRNA methyltransferase activity [GO:0008649];
TFIID-class transcription factor binding [GO:0001094]
Gene Ontology
(Cellular Component)
Complete annatation
box C/D snoRNP complex [GO:0031428];
Cajal body [GO:0015030];
dense fibrillar component [GO:0001651];
extracellular exosome [GO:0070062];
granular component [GO:0001652];
membrane [GO:0016020];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
small-subunit processome [GO:0032040]
Protein-protein interaction108399
Phylogenetic treeP22087
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.6922429323105810.03565127520626540.0751518908963402
AZA vs. DISU-0.2571338031435060.3087956251687420.866242136589333
AZA vs. IL70.2525138508659330.1881417039698720.952505417553304
AZA vs. SAHA-0.2827608203412240.2459372511412010.617805594723575
DISU vs. CD3-0.9611860613663280.008653902017930480.025638545404537
DISU vs. IL70.4998077796880420.04737474224749590.28774143407776
DISU vs. SAHA-0.0238991701342520.9345232208992370.984702472360512
DMSO vs. AZA0.04570466284033640.784376129506591
DMSO vs. CD3-0.6560749391702120.04156733535483640.082186936726432
DMSO vs. DISU0.3014127321084490.2163133317235790.728091984191312
DMSO vs. IL70.2136466749381520.2336669108238850.741355029737196
DMSO vs. SAHA-0.3355929300005620.1543811670458750.474226761959934
HIV vs. Mock in Activation0.021439177862110.9725825795116310.999983755607037
HIV vs. Mock in Latency-0.06150184482350770.7089783736989410.999834320637052
IL7 vs. CD3-0.4330565633956030.1794914679412940.297337608119477
SAHA vs. CD3-0.9991978107342640.005113953236750570.0145574754963791
SAHA vs. IL7-0.5379809932884990.02732623955793210.122593968778651
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
-2 0.026176162 -1.6 0.076462087 -1.3 0.399938745
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -1.73313 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.985 1.027 0.89 0.836 0.858
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
211623_s_at 1.42 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2IPX X-ray 1.8Å A=83-315.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03008 Ribosome biogenesis in eukaryotes - Homo sapiens (human)