Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001043
UniProt IDQ9H4H8
Primary gene name(s)FAM83D
Synonym gene name(s)C20orf129
Protein nameProtein FAM83D
Protein functionProbable proto-oncogene that regulates cell proliferation, growth, migration and epithelial to mesenchymal transition. Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC, PubMed:24344117. May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade, PubMed:25646692. May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22. {ECO:0000269|PubMed:18485706, ECO:0000269|PubMed:24344117, ECO:0000269|PubMed:25646692}.
Subcellular locationCytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:15561729, ECO:0000269|PubMed:18485706}. Note=Primarily cytoplasmic during interphase, but at prophase, associates with spindle microtubules, with a clear concentration toward the spindle poles. It persists on spindle microtubules through metaphase and anaphase. {ECO:0000269|PubMed:18485706}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9H4H8
Gene Ontology
(Biological Process)
Complete annatation
cell division [GO:0051301];
cell migration [GO:0016477];
cell proliferation [GO:0008283];
epithelial to mesenchymal transition [GO:0001837];
metaphase plate congression [GO:0051310];
mitotic nuclear division [GO:0007067];
positive regulation of cell cycle G1/S phase transition [GO:1902808];
protein localization to mitotic spindle [GO:1902480];
regulation of ERK1 and ERK2 cascade [GO:0070372];
regulation of protein catabolic process [GO:0042176];
regulation of TOR signaling [GO:0032006]
Gene Ontology
(Molecular Function)
Complete annatation
kinesin binding [GO:0019894];
microtubule binding [GO:0008017];
protein kinase binding [GO:0019901]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
mitotic spindle pole [GO:0097431]
Protein-protein interaction123547
Phylogenetic treeQ9H4H8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.4711205837560.5645567023285190.673553282474111
AZA vs. DISU-0.6571589798769350.2618523851528970.840925964890237
AZA vs. IL7-0.1003956829245020.8577474108272630.999311006273513
AZA vs. SAHA-0.8805979335910860.165363352199220.518952418388948
DISU vs. CD3-1.138741933649720.1355470224889860.236896906318722
DISU vs. IL70.546671095136430.1980307494197510.572232260270726
DISU vs. SAHA-0.2226492103904790.6679848171130980.898933766732129
DMSO vs. AZA-0.08093792829638620.8969909758484031
DMSO vs. CD3-0.567341714107690.4673309996323620.578072215244399
DMSO vs. DISU0.5722401256300660.2631593793381390.782674530614912
DMSO vs. IL7-0.01100694641084770.9818149779003550.997820041579181
DMSO vs. SAHA-0.805496967900790.15586057654430.476272252453193
HIV vs. Mock in Activation-0.6141029097800490.671229584952150.999983755607037
HIV vs. Mock in Latency0.06115007362411960.8313211315579750.999834320637052
IL7 vs. CD3-0.5613333973812110.4531784796890320.587036715740184
SAHA vs. CD3-1.379081742481640.08532928974718850.153444674237947
SAHA vs. IL7-0.7834888443896020.1112093618083460.306738361356155
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
2 4.94E-05 1.3 0.075425076 1.4 0.145389775
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.567729 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.095 1.154 1.836 2.632 1.087
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
5E0L X-ray 1.3Å C=384-394.
5E0M X-ray 1.6Å C=436-446.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found