Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000998
UniProt IDQ92800
Primary gene name(s)EZH1
Synonym gene name(s)KIAA0388
Protein nameHistone-lysine N-methyltransferase EZH1
Protein functionPolycomb group, PcG protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. {ECO:0000269|PubMed:19026781}.
Subcellular locationNucleus {ECO:0000269|PubMed:19026781}. Note=Colocalizes with trimethylated 'Lys-27' of histone H3.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q92800
Gene Ontology
(Biological Process)
Complete annatation
anatomical structure morphogenesis [GO:0009653];
hippocampus development [GO:0021766];
histone H3-K27 methylation [GO:0070734];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
histone-lysine N-methyltransferase activity [GO:0018024]
Gene Ontology
(Cellular Component)
Complete annatation
ESC/E(Z complex [GO:0035098];
nucleoplasm [GO:0005654]
Protein-protein interaction108445
Phylogenetic treeQ92800
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.290342381677310.000105468179527390.000505603181245811
AZA vs. DISU0.2701804261513160.2854021802390940.854449347551956
AZA vs. IL7-0.3568188066699060.06392905189053750.725604366826862
AZA vs. SAHA-0.05794362727904040.8122981385387520.95304910158264
DISU vs. CD31.547296764268563.01164075351412e-050.000196866750730581
DISU vs. IL7-0.6361681964752060.01185208465112740.130385983812284
DISU vs. SAHA-0.32621039396450.26305071663720.641183462791403
DMSO vs. AZA0.07958413666326860.6347880131545381
DMSO vs. CD31.357485980454783.02473529116831e-050.000154623811202751
DMSO vs. DISU-0.1925972688384520.4296831035989460.881718877024027
DMSO vs. IL7-0.4290473096151340.01726877503510570.272554298105888
DMSO vs. SAHA-0.1436376210410520.5425596698755250.834750181711649
HIV vs. Mock in Activation0.1968785535880050.7519317802248990.999983755607037
HIV vs. Mock in Latency0.05130347868804870.7562545489092260.999834320637052
IL7 vs. CD30.9402051592659060.003722717137235710.0130981595260396
SAHA vs. CD31.208319383802780.0007609060990828590.00282922647746822
SAHA vs. IL70.2953472591420050.2259050041125980.466365317417472
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.230445 0.119712
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00310 Lysine degradation - Homo sapiens (human)