Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000981
UniProt IDA0FGR8
Primary gene name(s)ESYT2
Synonym gene name(s)FAM62B, KIAA1228
Protein nameExtended synaptotagmin-2
Protein functionTethers the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane. Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport. Plays a role in FGF signaling via its role in the rapid internalization of FGFR1 that has been activated by FGF1 binding; this occurs most likely via the AP-2 complex. {ECO:0000269|PubMed:17360437, ECO:0000269|PubMed:20833364, ECO:0000269|PubMed:23791178, ECO:0000269|PubMed:24847877}.
Subcellular locationCell membrane;
Peripheral membrane protein. Endoplasmic reticulum membrane;
Multi-pass membrane protein. Note=Detected at sites of contact between the endoplasmic reticulum membrane and the cell membrane. Recruited to the cell membrane via the third C2 domain.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: A0FGR8
Gene Ontology
(Biological Process)
Complete annatation
endocytosis [GO:0006897];
lipid transport [GO:0006869]
Gene Ontology
(Molecular Function)
Complete annatation
calcium-dependent phospholipid binding [GO:0005544];
calcium ion binding [GO:0005509];
identical protein binding [GO:0042802];
phosphatidylcholine binding [GO:0031210];
phosphatidylethanolamine binding [GO:0008429];
phosphatidylinositol binding [GO:0035091]
Gene Ontology
(Cellular Component)
Complete annatation
extrinsic component of cytoplasmic side of plasma membrane [GO:0031234];
integral component of plasma membrane [GO:0005887];
intrinsic component of endoplasmic reticulum membrane [GO:0031227];
membrane [GO:0016020];
organelle membrane contact site [GO:0044232]
Protein-protein interaction121556
Phylogenetic treeA0FGR8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.106532019286960.0008772356703147240.00323743781170018
AZA vs. DISU0.02029423231607710.9359143368527150.995042856396371
AZA vs. IL7-0.008821687947972360.9633043607975090.999311006273513
AZA vs. SAHA0.0418942696653530.8635179057218890.967854034564039
DISU vs. CD31.113911426051540.002463474764205830.00896263032897014
DISU vs. IL7-0.03809089267460610.8795644099833960.976996113795713
DISU vs. SAHA0.02291096467074610.9372996156274880.985272223282263
DMSO vs. AZA0.01191745657755310.9430927544299071
DMSO vs. CD31.105563334712510.0006584832180507320.00236981597705071
DMSO vs. DISU-0.01060003604020570.9652699624787760.994294013509907
DMSO vs. IL7-0.01334391855957540.9406740141473090.988794358501781
DMSO vs. SAHA0.02364049388892090.9200751150376210.981674635498209
HIV vs. Mock in Activation0.209563278879380.7368153328960890.999983755607037
HIV vs. Mock in Latency0.1734502308315540.2915437436869580.999834320637052
IL7 vs. CD31.105432193091540.0006860227034110040.003072933197181
SAHA vs. CD31.123378136178420.001873159468222090.00613130173460183
SAHA vs. IL70.04692056990496970.8472773896533120.935256251584214
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.541752 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.037 0.969 1.146 1.241 1.003
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2DMG NMR - A=785-913.
4NPJ X-ray 2.1Å A/B=363-660.
4NPK X-ray 2.5Å A=363-660.
4P42 X-ray 2.4Å A/B=191-662.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found