Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000979
UniProt IDQ9UJM3
Primary gene name(s)ERRFI1
Synonym gene name(s)MIG6
Protein nameERBB receptor feedback inhibitor 1
Protein functionNegative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development, By similarity. {ECO:0000250}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:7641805}. Cell membrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250};
Cytoplasmic side {ECO:0000250}. Nucleus {ECO:0000250}. Note=Associated with the plasma membrane of basal skin keratinocytes. Translocates into the nucleus of differentiating suprabasal keratinocytes, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UJM3
Gene Ontology
(Biological Process)
Complete annatation
cellular hyperosmotic response [GO:0071474];
cellular response to dexamethasone stimulus [GO:0071549];
cellular response to epidermal growth factor stimulus [GO:0071364];
cellular response to insulin stimulus [GO:0032869];
cellular response to platelet-derived growth factor stimulus [GO:0036120];
lung alveolus development [GO:0048286];
lung epithelium development [GO:0060428];
lung vasculature development [GO:0060426];
negative regulation of cardiac muscle hypertrophy in response to stress [GO:1903243];
negative regulation of collagen biosynthetic process [GO:0032966];
negative regulation of epidermal growth factor-activated receptor activity [GO:0007175];
negative regulation of ERK1 and ERK2 cascade [GO:0070373];
negative regulation of interleukin-1 beta production [GO:0032691];
negative regulation of protein autophosphorylation [GO:0031953];
negative regulation of tumor necrosis factor biosynthetic process [GO:0042536];
regulation of keratinocyte differentiation [GO:0045616];
regulation of type B pancreatic cell proliferation [GO:0061469];
response to stress [GO:0006950];
skin morphogenesis [GO:0043589]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase activator activity [GO:0005096];
protein kinase binding [GO:0019901]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extrinsic component of cytoplasmic side of plasma membrane [GO:0031234];
nucleus [GO:0005634]
Protein-protein interaction119923
Phylogenetic treeQ9UJM3
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.4963714423292690.3057898943861520.424584549810049
AZA vs. DISU-0.1329395862860480.7944992785261750.981582011679663
AZA vs. IL7-0.319700634843180.3778508919745070.999311006273513
AZA vs. SAHA0.5020724106718940.1800929357314480.541580588230571
DISU vs. CD3-0.6430845552488880.2666415156946420.394828507620927
DISU vs. IL7-0.1951022471866810.6657633497252420.906789652524865
DISU vs. SAHA0.6353246906876330.1728453519879350.536550422206009
DMSO vs. AZA0.08784582805186240.8105697304186381
DMSO vs. CD3-0.4235454015690770.2955898395333780.405819824347975
DMSO vs. DISU0.2177671177724330.6306785534030830.945704536396344
DMSO vs. IL7-0.3996318934928560.1343449091945190.632122723648975
DMSO vs. SAHA0.4066297335209810.1469359781799560.46134835982294
HIV vs. Mock in Activation-0.1687816720641910.8346411434330330.999983755607037
HIV vs. Mock in Latency-0.002974629042709080.9951368862240770.999834320637052
IL7 vs. CD3-0.8080325815801280.05014229164739140.110002356394579
SAHA vs. CD3-0.02224354327778230.9562442732260990.970737702763039
SAHA vs. IL70.815659228701430.003479991376557970.0297209008556571
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
-1.6 0.016594991 -1.8 0.004982907 -1.4 0.233669259
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.467825 0.0580362
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.987 1.165 1.135 1.173 1.227
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2RF9 X-ray 3.5Å C/D=315-374.
2RFD X-ray 3.6Å C/D=340-364.
2RFE X-ray 2.9Å E/F=325-364.
4I21 X-ray 3.3Å C/D=315-374.
4R3P X-ray 2.9Å B=392-398.
4R3R X-ray 3.2Å B=392-398.
4ZJV X-ray 2.7Å C/D=330-399.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vif downregulates 23333304

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found