Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000974
UniProt IDQ2NKX8
Primary gene name(s)ERCC6L
Synonym gene name(s)PICH
Protein nameDNA excision repair protein ERCC-6-like
Protein functionDNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase. {ECO:0000269|PubMed:17218258}.
Subcellular locationChromosome, centromere. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha, TOP2A after anaphase onset.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q2NKX8
Gene Ontology
(Biological Process)
Complete annatation
cell division [GO:0051301];
mitotic nuclear division [GO:0007067];
sister chromatid cohesion [GO:0007062]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
DNA binding [GO:0003677];
helicase activity [GO:0004386]
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
condensed chromosome kinetochore [GO:0000777];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
membrane [GO:0016020];
nucleoplasm [GO:0005654];
plasma membrane [GO:0005886]
Protein-protein interaction120176
Phylogenetic treeQ2NKX8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.955322154399510.0001710470787462670.000773483819641085
AZA vs. DISU0.402761927103080.3555774327073410.891144071056453
AZA vs. IL70.2855261099755840.3652284880941940.999311006273513
AZA vs. SAHA-0.2026777478393140.4478209236583110.790855173834205
DISU vs. CD3-1.56601161570810.007951056234864360.0239708443368691
DISU vs. IL7-0.1236033730683190.7811452311766350.949641019244526
DISU vs. SAHA-0.6002954977621270.1552733509969690.507289111830767
DMSO vs. AZA-0.153165555669650.5236026688848531
DMSO vs. CD3-2.117650246899691.76930807863629e-059.60181059245522e-05
DMSO vs. DISU-0.5553050353597880.1643992325583550.673415720210493
DMSO vs. IL70.4434103506768630.08260915896317320.538064816065039
DMSO vs. SAHA-0.05701729612708040.8243420800441210.952182804864714
HIV vs. Mock in Activation0.05275594214909030.9601631264478820.999983755607037
HIV vs. Mock in Latency0.2989832591703150.1359085965027990.999834320637052
IL7 vs. CD3-1.657823848346840.001781578127613260.00697318284017519
SAHA vs. CD3-2.178567301376962.93625972821454e-050.000162861369540645
SAHA vs. IL7-0.4886453902947520.08351847870059230.257686117118719
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.437027 0.00108419
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.983 0.968 0.902 0.733 0.998
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found