Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000973
UniProt IDQ8IUD2
Primary gene name(s)ERC1
Synonym gene name(s)ELKS, KIAA1081, RAB6IP2
Protein nameELKS/Rab6-interacting/CAST family member 1
Protein functionRegulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex. May be involved in the organization of the cytomatrix at the nerve terminals active zone, CAZ which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport. {ECO:0000269|PubMed:15218148}.
Subcellular locationCytoplasm {ECO:0000250}. Membrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250}. Golgi apparatus membrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250}. Note=In neurons, localized closed to presynaptic membrane. Recruited on Golgi membranes by RAB6A in a GTP-dependent manner, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8IUD2
Gene Ontology
(Biological Process)
Complete annatation
I-kappaB phosphorylation [GO:0007252];
multicellular organism development [GO:0007275];
positive regulation of NF-kappaB transcription factor activity [GO:0051092];
protein transport [GO:0015031];
regulation of transcription, DNA-templated [GO:0006355];
retrograde transport, endosome to Golgi [GO:0042147]
Gene Ontology
(Molecular Function)
Complete annatation
leucine zipper domain binding [GO:0043522];
PDZ domain binding [GO:0030165];
Rab GTPase binding [GO:0017137]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
Golgi membrane [GO:0000139];
IkappaB kinase complex [GO:0008385];
presynaptic active zone [GO:0048786];
presynaptic membrane [GO:0042734];
synapse [GO:0045202]
Protein-protein interaction116714
Phylogenetic treeQ8IUD2
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.2451728425218870.4550996379280350.576069685298677
AZA vs. DISU0.2771815252685730.2751760223511050.846490201401045
AZA vs. IL70.1410044949697380.4671809509595710.999311006273513
AZA vs. SAHA0.1767397943512780.4707542001086160.805579821063475
DISU vs. CD30.5095279943040450.1610645622845660.270245682533119
DISU vs. IL7-0.1449377602928740.5672449768462090.860729340936148
DISU vs. SAHA-0.09949227089104770.7333802548693880.922217256665544
DMSO vs. AZA-0.01813411652353120.9148289645396041
DMSO vs. CD30.2144909610017860.503868406257560.611912740045961
DMSO vs. DISU-0.297519301552090.2254835534103430.738061935250622
DMSO vs. IL70.1665904335543180.3580761631133440.821745082570931
DMSO vs. SAHA0.1876348313612830.4282602675154590.764819349653453
HIV vs. Mock in Activation0.3199087465140590.6082098686354750.999983755607037
HIV vs. Mock in Latency-0.09521228570943930.568252307183050.999834320637052
IL7 vs. CD30.3944464764417160.2219610862424470.349338984330418
SAHA vs. CD30.3963921970829990.2631991083387660.37457838914583
SAHA vs. IL70.03121513213496460.8984394130556530.961219156596458
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.396356 0.00154878
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1 1.151 1.194 1.293 1.169
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04064 NF-kappa B signaling pathway - Homo sapiens (human)