Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000970
UniProt IDQ12929
Primary gene name(s)EPS8
Synonym gene name(s)unknown
Protein nameEpidermal growth factor receptor kinase substrate 8
Protein functionSignaling adapter that controls various cellular protrusions by regulating actin cytoskeleton dynamics and architecture. Depending on its association with other signal transducers, can regulate different processes. Together with SOS1 and ABI1, forms a trimeric complex that participates in transduction of signals from Ras to Rac by activating the Rac-specific guanine nucleotide exchange factor, GEF activity. Acts as a direct regulator of actin dynamics by binding actin filaments and has both barbed-end actin filament capping and actin bundling activities depending on the context. Displays barbed-end actin capping activity when associated with ABI1, thereby regulating actin-based motility process: capping activity is auto-inhibited and inhibition is relieved upon ABI1 interaction. Also shows actin bundling activity when associated with BAIAP2, enhancing BAIAP2-dependent membrane extensions and promoting filopodial protrusions. Involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration and cancer cell migration and invasion. Acts as a regulator of axonal filopodia formation in neurons: in the absence of neurotrophic factors, negatively regulates axonal filopodia formation via actin-capping activity. In contrast, it is phosphorylated in the presence of BDNF leading to inhibition of its actin-capping activity and stimulation of filopodia formation. Component of a complex with WHRN and MYO15A that localizes at stereocilia tips and is required for elongation of the stereocilia actin core. Indirectly involved in cell cycle progression; its degradation following ubiquitination being required during G2 phase to promote cell shape changes. {ECO:0000269|PubMed:15558031, ECO:0000269|PubMed:17115031}.
Subcellular locationCytoplasm, cell cortex {ECO:0000250}. Cell projection, ruffle membrane {ECO:0000250}. Cell projection, growth cone {ECO:0000250}. Cell projection, stereocilium {ECO:0000250, ECO:0000250|UniProtKB:Q08509}. Cell junction, synapse, synaptosome {ECO:0000250}. Note=Localizes at the tips of the stereocilia of the inner and outer hair cells, By similarity. Localizes to the midzone of dividing cells. {ECO:0000250, ECO:0000250|UniProtKB:Q08509}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q12929
Gene Ontology
(Biological Process)
Complete annatation
actin crosslink formation [GO:0051764];
actin cytoskeleton reorganization [GO:0031532];
actin filament bundle assembly [GO:0051017];
actin polymerization-dependent cell motility [GO:0070358];
adult locomotory behavior [GO:0008344];
barbed-end actin filament capping [GO:0051016];
behavioral response to ethanol [GO:0048149];
cell proliferation [GO:0008283];
dendritic cell migration [GO:0036336];
epidermal growth factor receptor signaling pathway [GO:0007173];
exit from mitosis [GO:0010458];
Rac protein signal transduction [GO:0016601];
regulation of actin filament length [GO:0030832];
regulation of cell shape [GO:0008360];
signal transduction [GO:0007165]
Gene Ontology
(Molecular Function)
Complete annatation
actin binding [GO:0003779];
Rac GTPase binding [GO:0048365];
SH3/SH2 adaptor activity [GO:0005070]
Gene Ontology
(Cellular Component)
Complete annatation
brush border [GO:0005903];
cell cortex [GO:0005938];
cell junction [GO:0030054];
extracellular exosome [GO:0070062];
growth cone [GO:0030426];
NMDA selective glutamate receptor complex [GO:0017146];
postsynaptic density [GO:0014069];
ruffle membrane [GO:0032587];
stereocilium [GO:0032420];
vesicle [GO:0031982]
Protein-protein interaction108373
Phylogenetic treeQ12929
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.304814649165490.0001535203794064130.000704800231012324
AZA vs. DISU0.05829909200914330.8567669878270010.989951370113767
AZA vs. IL7-0.1738939594875020.6268339753513670.999311006273513
AZA vs. SAHA0.4137028396712750.2062875602981540.574677265558005
DISU vs. CD31.349937612143540.0003582453142046970.0017176176419598
DISU vs. IL7-0.2416887687351590.4093518807853810.768682063133983
DISU vs. SAHA0.3550622016368880.235884150259290.614036521998339
DMSO vs. AZA-0.1102548951260030.7484846846255261
DMSO vs. CD31.176185872436240.0004587061252152580.00173149781417386
DMSO vs. DISU-0.1726356178755790.5230346951874590.915568922810432
DMSO vs. IL7-0.05573791569409010.8587837191923180.969594106441731
DMSO vs. SAHA0.5172507764016730.06111708989309740.283850743229236
HIV vs. Mock in Activation-0.05705266659945670.9298100071665230.999983755607037
HIV vs. Mock in Latency-0.4286290612668790.1641151173413780.999834320637052
IL7 vs. CD31.140414462230360.000730287566459320.00324568717223969
SAHA vs. CD31.691098654766715.76603978097445e-063.86884325464314e-05
SAHA vs. IL70.5830578048137420.05024085184089850.184669739246453
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.483011 0.00342251
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2E8M NMR - A=699-784.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18187620

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found