Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000965
UniProt IDP34913
Primary gene name(s)EPHX2
Synonym gene name(s)unknown
Protein nameBifunctional epoxide hydrolase 2 [Includes: Cytosolic epoxide hydrolase 2
Protein functionBifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides, alkene oxides, oxiranes and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phosphate. {ECO:0000269|PubMed:12574508, ECO:0000269|PubMed:12574510}.
Subcellular locationCytoplasm. Peroxisome.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P34913
Gene Ontology
(Biological Process)
Complete annatation
cellular calcium ion homeostasis [GO:0006874];
cholesterol homeostasis [GO:0042632];
dephosphorylation [GO:0016311];
drug metabolic process [GO:0017144];
epoxygenase P450 pathway [GO:0019373];
inflammatory response [GO:0006954];
phospholipid dephosphorylation [GO:0046839];
positive regulation of gene expression [GO:0010628];
positive regulation of vasodilation [GO:0045909];
reactive oxygen species metabolic process [GO:0072593];
regulation of blood pressure [GO:0008217];
regulation of cholesterol metabolic process [GO:0090181];
response to toxic substance [GO:0009636];
stilbene catabolic process [GO:0046272];
xenobiotic metabolic process [GO:0006805]
Gene Ontology
(Molecular Function)
Complete annatation
10-hydroxy-9-(phosphonooxyoctadecanoate phosphatase activity [GO:0033885];
epoxide hydrolase activity [GO:0004301];
lipid phosphatase activity [GO:0042577];
magnesium ion binding [GO:0000287];
phosphatase activity [GO:0016791];
protein homodimerization activity [GO:0042803];
receptor binding [GO:0005102];
toxic substance binding [GO:0015643]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
peroxisome [GO:0005777]
Protein-protein interaction108367
Phylogenetic treeP34913
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.40761463007480.02458391391854210.0552368441532896
AZA vs. DISU-0.7975359245504870.01172039543397540.263756634321933
AZA vs. IL7-0.4270762309584060.2597012093401750.999311006273513
AZA vs. SAHA-0.6435225178036990.4427961945342770.788183866617569
DISU vs. CD30.5980854108195090.2987613247855490.430095330896511
DISU vs. IL70.3594477356544190.2078641709080570.587137413610448
DISU vs. SAHA0.1488280608909430.8598169708162520.962605197100489
DMSO vs. AZA0.1155447757603670.7883945298887971
DMSO vs. CD31.504139631915710.01715343370572840.0393302488889261
DMSO vs. DISU0.9051929361673960.006264867142536380.169760733783758
DMSO vs. IL7-0.5331393010653930.1747994148620680.684776955532741
DMSO vs. SAHA-0.7639473287125270.3645381841653650.712898605095376
HIV vs. Mock in Activation-0.1623914837492140.8959141930484190.999983755607037
HIV vs. Mock in Latency-0.4246322933850430.02092372785420850.540075811086196
IL7 vs. CD30.9887999359376610.1019807455019390.192840211741685
SAHA vs. CD30.7448419393816950.4146124440838630.529688837940303
SAHA vs. IL7-0.2224549056242470.7870189466016540.906719932824315
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander -1.005 1.69E-11 2.18E-09
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.217373887
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -1.1456 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
209368_at 3.04 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB02029 N-Cyclohexyl-N&,39;-(4-Iodophenyl)Urea experimental unknown unknown
DB03677 N-Cyclohexyl-N&,39;-Decylurea experimental unknown unknown
DB04213 N-Cyclohexyl-N&,39;-(Propyl)Phenyl Urea experimental unknown unknown
DB08256 N-[(CYCLOHEXYLAMINO)CARBONYL]GLYCINE experimental unknown unknown
DB08257 4-{[(CYCLOHEXYLAMINO)CARBONYL]AMINO}BUTANOIC ACID experimental unknown unknown
DB08258 6-{[(CYCLOHEXYLAMINO)CARBONYL]AMINO}HEXANOIC ACID experimental unknown unknown
DB08259 7-{[(CYCLOHEXYLAMINO)CARBONYL]AMINO}HEPTANOIC ACID experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1S8O X-ray 2.6Å A=1-555.
1VJ5 X-ray 2.3Å A=1-555.
1ZD2 X-ray 3.0Å P=1-555.
1ZD3 X-ray 2.3Å A=1-555.
1ZD4 X-ray 2.7Å A=1-555.
1ZD5 X-ray 2.6Å A=1-555.
3ANS X-ray 1.9Å A/B=230-555.
3ANT X-ray 2.4Å A/B=230-555.
3I1Y X-ray 2.4Å A=1-555.
3I28 X-ray 1.9Å A=1-555.
3KOO X-ray 2.7Å A=1-555.
3OTQ X-ray 3.0Å A=1-555.
3PDC X-ray 2.6Å A/B=226-548.
3WK4 X-ray 2.1Å A=1-555.
3WK5 X-ray 2.7Å A=1-555.
3WK6 X-ray 2.1Å A=1-555.
3WK7 X-ray 2.2Å A=1-555.
3WK8 X-ray 2.2Å A=1-555.
3WK9 X-ray 2.2Å A=1-555.
3WKA X-ray 2.0Å A=1-555.
3WKB X-ray 2.2Å A=1-555.
3WKC X-ray 2.2Å A=1-555.
3WKD X-ray 2.4Å A=1-555.
3WKE X-ray 2.7Å A=1-555.
4C4X X-ray 2.1Å A/B=230-555.
4C4Y X-ray 2.4Å A=230-555.
4C4Z X-ray 2.0Å A/B=230-555.
4HAI X-ray 2.5Å A=1-555.
4J03 X-ray 2.9Å A=1-555.
4JNC X-ray 1.9Å A=238-549.
4OCZ X-ray 2.9Å A=1-555.
4OD0 X-ray 2.9Å A=1-555.
4X6X X-ray 1.8Å A/B=230-555.
4X6Y X-ray 2.1Å A/B=230-555.
4Y2J X-ray 2.1Å A=1-555.
4Y2P X-ray 2.0Å A=1-555.
4Y2Q X-ray 2.4Å A=1-555.
4Y2R X-ray 2.4Å A=1-555.
4Y2S X-ray 2.3Å A=1-555.
4Y2T X-ray 2.4Å A=1-555.
4Y2U X-ray 2.7Å A=1-555.
4Y2V X-ray 2.4Å A=1-555.
4Y2X X-ray 2.5Å A=1-555.
4Y2Y X-ray 2.3Å A=1-555.
5AHX X-ray 2.0Å A=1-548.
5AI0 X-ray 1.7Å A=1-548.
5AI4 X-ray 1.9Å A=1-548.
5AI5 X-ray 2.2Å A=1-548.
5AI6 X-ray 2.3Å A=1-548.
5AI8 X-ray 1.8Å A=1-548.
5AI9 X-ray 1.8Å A=1-548.
5AIA X-ray 2.2Å A=1-548.
5AIB X-ray 1.9Å A=1-548.
5AIC X-ray 1.8Å A=1-548.
5AK3 X-ray 2.2Å A=1-548.
5AK4 X-ray 1.7Å A=1-548.
5AK5 X-ray 2.2Å A=1-548.
5AK6 X-ray 2.1Å A=1-548.
5AKE X-ray 2.2Å A=1-548.
5AKG X-ray 2.5Å A=1-548.
5AKH X-ray 2.1Å A=1-548.
5AKI X-ray 1.8Å A=1-548.
5AKJ X-ray 2.0Å A=1-548.
5AKK X-ray 1.9Å A=1-548.
5AKL X-ray 2.0Å A=1-548.
5AKX X-ray 2.0Å A=1-548.
5AKY X-ray 2.1Å A=1-548.
5AKZ X-ray 2.1Å A=1-548.
5ALD X-ray 2.2Å A=1-548.
5ALE X-ray 1.9Å A=1-548.
5ALF X-ray 2.3Å A=1-548.
5ALG X-ray 2.4Å A=1-548.
5ALH X-ray 1.9Å A=1-548.
5ALI X-ray 1.8Å A=1-548.
5ALJ X-ray 2.1Å A=1-548.
5ALK X-ray 2.3Å A=1-548.
5ALL X-ray 2.2Å A=1-548.
5ALM X-ray 2.0Å A=1-548.
5ALN X-ray 2.0Å A=1-548.
5ALO X-ray 2.0Å A=1-548.
5ALP X-ray 2.0Å A=1-548.
5ALQ X-ray 2.7Å A=1-548.
5ALR X-ray 2.6Å A=1-548.
5ALS X-ray 2.5Å A=1-548.
5ALT X-ray 2.1Å A=1-548.
5ALU X-ray 1.8Å A=1-548.
5ALV X-ray 1.8Å A=1-548.
5ALW X-ray 2.2Å A=1-548.
5ALX X-ray 2.2Å A=1-548.
5ALY X-ray 1.9Å A=1-548.
5ALZ X-ray 2.3Å A=1-548.
5AM0 X-ray 1.8Å A=1-548.
5AM1 X-ray 2.1Å A=1-548.
5AM2 X-ray 1.7Å A=1-548.
5AM3 X-ray 2.2Å A=1-548.
5AM4 X-ray 1.8Å A=1-548.
5AM5 X-ray 2.2Å A=1-548.
5FP0 X-ray 2.3Å A=1-548.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00590 Arachidonic acid metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04146 Peroxisome - Homo sapiens (human)