Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000962
UniProt IDP21709
Primary gene name(s)EPHA1
Synonym gene name(s)EPH, EPHT, EPHT1
Protein nameEphrin type-A receptor 1
Protein functionReceptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis. {ECO:0000269|PubMed:17634955, ECO:0000269|PubMed:19118217, ECO:0000269|PubMed:20043122}.
Subcellular locationCell membrane {ECO:0000269|PubMed:19118217};
Single-pass type I membrane protein {ECO:0000269|PubMed:19118217}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P21709
Gene Ontology
(Biological Process)
Complete annatation
activation of GTPase activity [GO:0090630];
angiogenesis [GO:0001525];
cell surface receptor signaling pathway [GO:0007166];
ephrin receptor signaling pathway [GO:0048013];
negative regulation of cell migration [GO:0030336];
negative regulation of protein kinase activity [GO:0006469];
peptidyl-tyrosine phosphorylation [GO:0018108];
positive regulation of angiogenesis [GO:0045766];
positive regulation of cell-matrix adhesion [GO:0001954];
positive regulation of cell migration [GO:0030335];
positive regulation of cell proliferation [GO:0008284];
positive regulation of stress fiber assembly [GO:0051496];
protein autophosphorylation [GO:0046777];
regulation of GTPase activity [GO:0043087];
somatic stem cell population maintenance [GO:0035019];
substrate adhesion-dependent cell spreading [GO:0034446]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
protein kinase activity [GO:0004672];
protein kinase binding [GO:0019901];
transmembrane-ephrin receptor activity [GO:0005005]
Gene Ontology
(Cellular Component)
Complete annatation
integral component of plasma membrane [GO:0005887];
plasma membrane [GO:0005886]
Protein-protein interaction108355
Phylogenetic treeP21709
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.703292065136250.0001758913145855080.000792062710915081
AZA vs. DISU-0.8658357503766820.004034064847578510.164349982465942
AZA vs. IL7-0.2141444260713750.376207346020970.999311006273513
AZA vs. SAHA1.724537922556060.0002233039530670220.00694558221220542
DISU vs. CD30.8257352767730750.07507335719278930.148921672104765
DISU vs. IL70.6434041615044570.02673120853272660.20893162391058
DISU vs. SAHA2.591174374510641.60275763549933e-072.17614056978472e-05
DMSO vs. AZA0.1318443701368260.6586111570123571
DMSO vs. CD31.812567329090168.34209985742795e-050.00038233566885352
DMSO vs. DISU0.9923852858993040.001863725126973080.0888808153907732
DMSO vs. IL7-0.3347747682307520.2085104451025030.717319707877736
DMSO vs. SAHA1.586142807525650.0009135984950475740.0168529289092558
HIV vs. Mock in Activationunknownunknownunknown
HIV vs. Mock in Latency-0.7106016749802390.0003679170834924860.02965566171847
IL7 vs. CD31.498126160650090.0004638107301687680.00219981722335866
SAHA vs. CD33.411515793637553.21760121879677e-083.85140987825793e-07
SAHA vs. IL71.930242724381191.90123724043145e-050.000517314984878406
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
-1.1 0.312515992 -1.3 0.021486732 -1.8 0.000497671
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.993976 0.551355
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2K1K NMR - A/B=536-573.
2K1L NMR - A/B=536-573.
3HIL X-ray 2.0Å A/B=911-974.
3KKA X-ray 2.4Å A/B=911-974.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04360 Axon guidance - Homo sapiens (human)