Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000961
UniProt IDP11171
Primary gene name(s)EPB41
Synonym gene name(s)E41P
Protein nameProtein 4.1
Protein functionProtein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes.
Subcellular locationCytoplasm, cytoskeleton {ECO:0000269|PubMed:12427749}. Cytoplasm, cell cortex {ECO:0000269|PubMed:12427749}. Nucleus {ECO:0000269|PubMed:12427749}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P11171
Gene Ontology
(Biological Process)
Complete annatation
actin cytoskeleton organization [GO:0030036];
actomyosin structure organization [GO:0031032];
blood circulation [GO:0008015];
cortical actin cytoskeleton organization [GO:0030866];
positive regulation of protein binding [GO:0032092]
Gene Ontology
(Molecular Function)
Complete annatation
1-phosphatidylinositol binding [GO:0005545];
spectrin binding [GO:0030507];
structural constituent of cytoskeleton [GO:0005200]
Gene Ontology
(Cellular Component)
Complete annatation
cell junction [GO:0030054];
cortical cytoskeleton [GO:0030863];
cytoplasm [GO:0005737];
extrinsic component of membrane [GO:0019898];
nucleus [GO:0005634];
plasma membrane [GO:0005886];
protein complex [GO:0043234];
spectrin [GO:0008091];
spectrin-associated cytoskeleton [GO:0014731]
Protein-protein interaction108349
Phylogenetic treeP11171
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.125167565253349.38596533828218e-101.36549488010221e-08
AZA vs. DISU0.3058050004637570.2263374196340590.81393616321201
AZA vs. IL7-0.4512908128414530.01877627014583980.443596739668094
AZA vs. SAHA-0.1724788432898370.4791856698395750.808696838080511
DISU vs. CD32.417766041868033.51172868562344e-107.85397599511687e-09
DISU vs. IL7-0.7661452580255960.002435948639179060.0482946825531614
DISU vs. SAHA-0.4768577180416020.1017154185657250.407808745199266
DMSO vs. AZA-0.04223778291604320.8000326875614761
DMSO vs. CD32.069547284664592.00089578239471e-092.44274675541137e-08
DMSO vs. DISU-0.3504320182337010.1506882178963080.648820412484962
DMSO vs. IL7-0.4015658241969890.02527990500832960.323235426633221
DMSO vs. SAHA-0.1363951704262270.5624382156752250.845433839681773
HIV vs. Mock in Activation0.1771605556986130.8151505777005510.999983755607037
HIV vs. Mock in Latency0.1364216816941570.4083959228260620.999834320637052
IL7 vs. CD31.681312227516136.72580021565672e-076.83600719154374e-06
SAHA vs. CD31.927597921064271.76401880991328e-071.72860297951923e-06
SAHA vs. IL70.2745713283747410.2587823280893250.504120549705154
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.3143 0.04082

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander -0.788 6.36E-09 2.54E-07
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.573317 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.95 1.113 1.036 1.024 1.253
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1GG3 X-ray 2.8Å A/B/C=210-488.
2RQ1 NMR - A=292-396.
3QIJ X-ray 1.8Å A/B=211-488.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) incorporates 25631074
HIV-1 virus replication enhanced by expression of human gene 19460752

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found