Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000960
UniProt IDQ9NPA8
Primary gene name(s)ENY2
Synonym gene name(s)unknown
Protein nameTranscription and mRNA export factor ENY2
Protein functionInvolved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. The transcription regulatory histone acetylation, HAT complex SAGA is a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles, mRNPs to the nuclear entrance of the nuclear pore complex, nuclear basket. TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. {ECO:0000269|PubMed:18206972, ECO:0000269|PubMed:21746879}.
Subcellular locationNucleus, nucleoplasm {ECO:0000255|HAMAP-Rule:MF_03046, ECO:0000269|PubMed:22307388}. Nucleus, nuclear pore complex {ECO:0000255|HAMAP-Rule:MF_03046, ECO:0000269|PubMed:22307388}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NPA8
Gene Ontology
(Biological Process)
Complete annatation
histone deubiquitination [GO:0016578];
negative regulation of insulin secretion involved in cellular response to glucose stimulus [GO:0061179];
poly(A+ mRNA export from nucleus [GO:0016973];
positive regulation of transcription, DNA-templated [GO:0045893];
protein transport [GO:0015031];
regulation of transcription from RNA polymerase II promoter [GO:0006357];
transcription elongation from RNA polymerase II promoter [GO:0006368]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
ligand-dependent nuclear receptor transcription coactivator activity [GO:0030374];
transcription coactivator activity [GO:0003713]
Gene Ontology
(Cellular Component)
Complete annatation
DUBm complex [GO:0071819];
nuclear pore [GO:0005643];
SAGA complex [GO:0000124];
transcription export complex 2 [GO:0070390]
Protein-protein interaction121267
Phylogenetic treeQ9NPA8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.256975279397410.0001555839777076520.000713187868616097
AZA vs. DISU0.07464717305076510.7688722418854760.976327198566393
AZA vs. IL70.1408908053720620.4661280669108760.999311006273513
AZA vs. SAHA-0.08837017647459320.7188100081637140.921294283059249
DISU vs. CD3-1.195241904043610.001143070311627640.00465818920721141
DISU vs. IL70.05760937874477420.8198510340388960.961243368243154
DISU vs. SAHA-0.1621538399041320.581837011388580.865212653639199
DMSO vs. AZA-0.1749720810326430.3008197964916221
DMSO vs. CD3-1.441686430465829.83949790145378e-065.68156519300119e-05
DMSO vs. DISU-0.2510010103044930.3063535681623410.811664604818297
DMSO vs. IL70.3228752464869210.07460746459108310.517988567378705
DMSO vs. SAHA0.07886316176599120.7394187232280580.9244881016899
HIV vs. Mock in Activation-0.0937128019429470.8801707744637570.999983755607037
HIV vs. Mock in Latency-0.0360577314529150.8285547584988550.999834320637052
IL7 vs. CD3-1.107350013795430.0006548971229143330.00295723916527494
SAHA vs. CD3-1.370230019683470.0001464284621376380.000670180173312743
SAHA vs. IL7-0.2330444203443330.3413077325049750.590839722110918
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.226901 0.112878
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.946 1.047 0.938 0.784 1.031
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4DHX X-ray 2.1Å B/C/E/F=1-101.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found