Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000959
UniProt IDO75356
Primary gene name(s)ENTPD5
Synonym gene name(s)CD39L4, PCPH
Protein nameEctonucleoside triphosphate diphosphohydrolase 5
Protein functionUridine diphosphatase, UDPase that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. The nucleotide hydrolyzing preference is GDP > IDP > UDP, but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase, PI3K signaling. {ECO:0000269|PubMed:10400613}.
Subcellular locationEndoplasmic reticulum {ECO:0000250}. Secreted {ECO:0000269|PubMed:10400613}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O75356
Gene Ontology
(Biological Process)
Complete annatation
'de novo' posttranslational protein folding [GO:0051084];
ATP metabolic process [GO:0046034];
cell growth [GO:0016049];
cell proliferation [GO:0008283];
positive regulation of glycolytic process [GO:0045821];
protein N-linked glycosylation [GO:0006487];
regulation of phosphatidylinositol 3-kinase signaling [GO:0014066]
Gene Ontology
(Molecular Function)
Complete annatation
guanosine-diphosphatase activity [GO:0004382];
uridine-diphosphatase activity [GO:0045134]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum [GO:0005783];
extracellular exosome [GO:0070062]
Protein-protein interaction107395
Phylogenetic treeO75356
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.5507633513387510.098585042492770.174010277602884
AZA vs. DISU-0.4962737296736250.05331258451734320.513649338965264
AZA vs. IL7-0.01946959081939580.9204995506574770.999311006273513
AZA vs. SAHA0.01381531827047970.9552331484799180.990591096833318
DISU vs. CD30.04182669830445630.9109334151683890.941991514045983
DISU vs. IL70.4675189486667720.06755891175277760.34503852668197
DISU vs. SAHA0.5116849267873250.08271146694158280.365341300784465
DMSO vs. AZA0.07496703350578890.6607579794723971
DMSO vs. CD30.6123650027524420.06062754396297530.113226412295023
DMSO vs. DISU0.5690205738534010.0214930404987890.302873490922657
DMSO vs. IL7-0.08705329938991810.6333781503116510.920647261241455
DMSO vs. SAHA-0.06677561078571580.7787340243959730.93747907034681
HIV vs. Mock in Activation0.09142696952348930.887059181523220.999983755607037
HIV vs. Mock in Latency-0.06574829945570430.6943870703265160.999834320637052
IL7 vs. CD30.538807260372280.09879844043303280.187957704486571
SAHA vs. CD30.5404181738147260.1331368492680890.219131970125612
SAHA vs. IL70.02998582938755510.9027998262812720.963318792479924
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0337823 0.917437
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00230 Purine metabolism - Homo sapiens (human)
hsa00240 Pyrimidine metabolism - Homo sapiens (human)