Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000954
UniProt IDP06733
Primary gene name(s)ENO1
Synonym gene name(s)ENO1L1, MBPB1, MPB1
Protein nameAlpha-enolase
Protein functionMultifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.; FUNCTION: MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Subcellular locationCytoplasm {ECO:0000269|PubMed:10802057}. Cell membrane {ECO:0000269|PubMed:10802057}. Cytoplasm, myofibril, sarcomere, M line {ECO:0000269|PubMed:10802057}. Note=Can translocate to the plasma membrane in either the homodimeric, alpha/alpha or heterodimeric, alpha/gamma form. ENO1 is localized to the M line.;
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P06733
Gene Ontology
(Biological Process)
Complete annatation
canonical glycolysis [GO:0061621];
gluconeogenesis [GO:0006094];
negative regulation of cell growth [GO:0030308];
negative regulation of transcription, DNA-templated [GO:0045892];
response to virus [GO:0009615];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
GTPase binding [GO:0051020];
magnesium ion binding [GO:0000287];
phosphopyruvate hydratase activity [GO:0004634];
poly(A RNA binding [GO:0044822];
transcription corepressor activity [GO:0003714];
transcription factor activity, sequence-specific DNA binding [GO:0003700]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
extracellular space [GO:0005615];
extrinsic component of plasma membrane [GO:0019897];
M band [GO:0031430];
membrane [GO:0016020];
myelin sheath [GO:0043209];
nucleus [GO:0005634];
phosphopyruvate hydratase complex [GO:0000015]
Protein-protein interaction108338
Phylogenetic treeP06733
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.6667021882786.66984613872934e-075.37472869401634e-06
AZA vs. DISU0.2596477206077340.5348050319966120.940949166421897
AZA vs. IL70.602130073137460.001726524196850710.121041391716887
AZA vs. SAHA-0.1342791506948940.5840360141278640.862014968859632
DISU vs. CD3-1.419853617824340.00101362217163770.00420108347958939
DISU vs. IL70.3331733129406540.4081824556539640.768016789688406
DISU vs. SAHA-0.3909874727303320.3653495825540790.738446417122504
DMSO vs. AZA0.09995623440936380.5759063828813891
DMSO vs. CD3-1.578162454928521.3664786701062e-069.55266875412987e-06
DMSO vs. DISU-0.1615003705958420.6872863068709480.957954410639349
DMSO vs. IL70.509542146387610.004514007193419320.13528560326202
DMSO vs. SAHA-0.2393248710583170.3089265788984450.660707227613834
HIV vs. Mock in Activation0.03371396698500940.9567679354430050.999983755607037
HIV vs. Mock in Latency0.1065856945840920.7588649196961210.999834320637052
IL7 vs. CD3-1.058475171132070.001095451579704810.00458914443048655
SAHA vs. CD3-1.823514152222655.27619034795812e-074.60184742022419e-06
SAHA vs. IL7-0.7382022550810680.002503791290551560.0232976234035643
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.220687 0.120091
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.044 0.844 0.801 0.832 0.705
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2PSN X-ray 2.2Å A/B/C/D=1-434.
3B97 X-ray 2.2Å A/B/C/D=2-434.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 complexes with 23125841
Tat inhibits 9093905
retropepsin cleaves 22944692
Gag-Pol complexes with 23125841
Nef downregulates 25874870
Pr55(Gag) complexes with 23125841
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00010 Glycolysis / Gluconeogenesis - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01200 Carbon metabolism - Homo sapiens (human)
hsa01230 Biosynthesis of amino acids - Homo sapiens (human)
hsa03018 RNA degradation - Homo sapiens (human)
hsa04066 HIF-1 signaling pathway - Homo sapiens (human)