Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000938
UniProt IDQ15717
Primary gene name(s)ELAVL1
Synonym gene name(s)HUR
Protein nameELAV-like protein 1
Protein functionRNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability, PubMed:14517288, PubMed:18285462. Involved in embryonic stem cells, ESCs differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine, m6A, stabilizing them, promoting ESCs differentiation, By similarity. Binds to poly-U elements and AU-rich elements, AREs in the 3'-UTR of target mRNAs, PubMed:8626503, PubMed:17632515, PubMed:18285462, PubMed:23519412. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro, PubMed:8626503. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA, By similarity. {ECO:0000250|UniProtKB:P70372, ECO:0000269|PubMed:14517288, ECO:0000269|PubMed:17632515, ECO:0000269|PubMed:18285462, ECO:0000269|PubMed:19029303, ECO:0000269|PubMed:23519412, ECO:0000269|PubMed:8626503}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:14517288, ECO:0000269|PubMed:17632515, ECO:0000269|PubMed:18285462, ECO:0000269|PubMed:19029303}. Nucleus {ECO:0000269|PubMed:14517288, ECO:0000269|PubMed:17632515, ECO:0000269|PubMed:18285462}. Note=Translocates into the cytoplasm following phosphorylation by MAPKAPK2, PubMed:14517288. Likewise, phosphorylation by PRKCD promotes translocation from the nucleus into the cytoplasm, where it is associated with free and cytoskeleton-bound polysomes, PubMed:18285462. {ECO:0000269|PubMed:14517288, ECO:0000269|PubMed:18285462}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q15717
Gene Ontology
(Biological Process)
Complete annatation
3'-UTR-mediated mRNA stabilization [GO:0070935];
mRNA splicing, via spliceosome [GO:0000398];
mRNA stabilization [GO:0048255];
multicellular organism development [GO:0007275];
negative regulation of gene silencing by miRNA [GO:0060965];
positive regulation of translation [GO:0045727];
regulation of mRNA stability [GO:0043488];
regulation of stem cell population maintenance [GO:2000036]
Gene Ontology
(Molecular Function)
Complete annatation
AU-rich element binding [GO:0017091];
double-stranded RNA binding [GO:0003725];
mRNA 3'-UTR AU-rich region binding [GO:0035925];
mRNA 3'-UTR binding [GO:0003730];
mRNA binding [GO:0003729];
nucleotide binding [GO:0000166];
poly(A RNA binding [GO:0044822];
protein homodimerization activity [GO:0042803];
protein kinase binding [GO:0019901];
RNA binding [GO:0003723]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
membrane [GO:0016020];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction108309
Phylogenetic treeQ15717
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.519659151729975.49045643649659e-063.60105132336278e-05
AZA vs. DISU-0.109160906454490.6673229157891150.962557690454146
AZA vs. IL70.2036432690262290.2913598084748970.999311006273513
AZA vs. SAHA0.1586164641264780.5174779331669190.831689476712418
DISU vs. CD3-1.640451182729861.03085763286659e-057.65823440637051e-05
DISU vs. IL70.3034962637006410.2294738280778640.613096835926285
DISU vs. SAHA0.2693146283806730.3609440090161380.736101104178919
DMSO vs. AZA-0.06196373120380020.7129993550385191
DMSO vs. CD3-1.591889103500821.13930056189204e-068.10366523366554e-06
DMSO vs. DISU0.04564783343331790.8520697646131670.982981807603187
DMSO vs. IL70.2728035977852550.1308200207490570.62890965394892
DMSO vs. SAHA0.2140866633090770.3665254531733420.714421249970957
HIV vs. Mock in Activation0.1662152171309970.7891952780211030.999983755607037
HIV vs. Mock in Latency-0.05650584523307550.7334540421699240.999834320637052
IL7 vs. CD3-1.309191932096686.01260225023825e-050.000373569471219461
SAHA vs. CD3-1.385290236806750.0001219617521389660.000571683858393225
SAHA vs. IL7-0.04769994554266930.8457953311708270.934681236487055
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0326001 0.866187
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.015 0.935 0.873 0.815 0.947
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3HI9 X-ray 2.0Å A/B/C/D=18-99.
4ED5 X-ray 2.0Å A/B=18-186.
4EGL X-ray 2.9Å A=18-186.
4FXV X-ray 1.9Å A/B/C/D=20-99.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 complexes with 23125841
reverse transcriptase interacts with 18544151
Gag-Pol complexes with 23125841
nucleocapsid upregulates 18051367
Pr55(Gag) complexes with 23125841
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04152 AMPK signaling pathway - Homo sapiens (human)
hsa04657 IL-17 signaling pathway - Homo sapiens (human)