Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000931
UniProt IDQ04637
Primary gene name(s)EIF4G1
Synonym gene name(s)EIF4F, EIF4G, EIF4GI
Protein nameEukaryotic translation initiation factor 4 gamma 1
Protein functionComponent of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q04637
Gene Ontology
(Biological Process)
Complete annatation
behavioral fear response [GO:0001662];
cap-dependent translational initiation [GO:0002191];
cellular macromolecule biosynthetic process [GO:0034645];
developmental process [GO:0032502];
mitochondrion organization [GO:0007005];
negative regulation of autophagy [GO:0010507];
negative regulation of neuron death [GO:1901215];
negative regulation of peptidyl-threonine phosphorylation [GO:0010801];
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [GO:0000184];
nuclear-transcribed mRNA poly(A tail shortening [GO:0000289];
positive regulation of cell growth [GO:0030307];
positive regulation of cell proliferation [GO:0008284];
positive regulation of cellular protein metabolic process [GO:0032270];
positive regulation of energy homeostasis [GO:2000507];
positive regulation of eukaryotic translation initiation factor 4F complex assembly [GO:1905537];
positive regulation of G1/S transition of mitotic cell cycle [GO:1900087];
positive regulation of miRNA mediated inhibition of translation [GO:1905618];
positive regulation of mRNA cap binding [GO:1905612];
positive regulation of neuron differentiation [GO:0045666];
positive regulation of peptidyl-serine phosphorylation [GO:0033138];
regulation of cellular response to stress [GO:0080135];
regulation of gene silencing by miRNA [GO:0060964];
regulation of mRNA stability [GO:0043488];
regulation of presynapse assembly [GO:1905606];
regulation of translational initiation [GO:0006446];
translation [GO:0006412];
translational initiation [GO:0006413];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
eukaryotic initiation factor 4E binding [GO:0008190];
poly(A RNA binding [GO:0044822];
translation factor activity, RNA binding [GO:0008135];
translation initiation factor activity [GO:0003743]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
eukaryotic translation initiation factor 4F complex [GO:0016281];
membrane [GO:0016020]
Protein-protein interaction108296
Phylogenetic treeQ04637
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.664189442287.4273786587753e-075.92617528560409e-06
AZA vs. DISU0.08699347570632680.7303768932057590.974560663114398
AZA vs. IL70.3610930652043370.05982982269887530.703798825350824
AZA vs. SAHA-0.2099903185535730.3885182370414480.75136169098096
DISU vs. CD3-1.589857385161381.81861540877737e-050.000126330915538756
DISU vs. IL70.2648633378771190.2922639875925690.677686348192229
DISU vs. SAHA-0.2945827405192540.3119339355810050.694271084930758
DMSO vs. AZA0.04480773700706080.7883308401875341
DMSO vs. CD3-1.630917346880396.84916634963173e-075.08539630641705e-06
DMSO vs. DISU-0.04413173687103390.8562318847416310.983807945138555
DMSO vs. IL70.3237647140911360.07114653922314060.510394761039365
DMSO vs. SAHA-0.2605040488288530.2683186707501390.617325359573884
HIV vs. Mock in Activation0.3156336065132840.6116555404688310.999983755607037
HIV vs. Mock in Latency-0.06310641999333220.7011842582179230.999834320637052
IL7 vs. CD3-1.296829277083927.15045910260059e-050.000435118571067819
SAHA vs. CD3-1.89774960144742.14937318943065e-072.06312445635158e-06
SAHA vs. IL7-0.5734689311825150.0186490146098690.095911044808094
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.272844 0.046998
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.959 0.966 0.828 0.802 0.854
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1LJ2 X-ray 2.3Å C/D=172-199.
1UG3 X-ray 2.2Å A/B=1233-1571.
2W97 X-ray 2.2Å E/F=609-622.
4AZA X-ray 2.1Å B/D=609-620.
4F02 X-ray 2.0Å C/F=178-203.
5EHC X-ray 2.4Å B=609-622.
5EI3 X-ray 1.7Å B=609-622.
5EIR X-ray 2.6Å B=609-622.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) incorporates 25631074
retropepsin cleaves 11606767
Tat co-localizes with 23840900
Pr55(Gag) requires 18631141

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03013 RNA transport - Homo sapiens (human)
hsa05416 Viral myocarditis - Homo sapiens (human)