Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000930
UniProt IDP06730
Primary gene name(s)EIF4E
Synonym gene name(s)EIF4EL1, EIF4F
Protein nameEukaryotic translation initiation factor 4E
Protein functionRecognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. {ECO:0000269|PubMed:16271312, ECO:0000269|PubMed:22578813}.
Subcellular locationCytoplasm, P-body {ECO:0000269|PubMed:20616046}. Cytoplasm {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P06730
Gene Ontology
(Biological Process)
Complete annatation
behavioral fear response [GO:0001662];
cellular response to dexamethasone stimulus [GO:0071549];
G1/S transition of mitotic cell cycle [GO:0000082];
lung development [GO:0030324];
mRNA export from nucleus [GO:0006406];
negative regulation of neuron differentiation [GO:0045665];
negative regulation of translation [GO:0017148];
nuclear-transcribed mRNA poly(A tail shortening [GO:0000289];
positive regulation of mitotic cell cycle [GO:0045931];
regulation of translation [GO:0006417];
RNA export from nucleus [GO:0006405];
stem cell population maintenance [GO:0019827];
translational initiation [GO:0006413];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
eukaryotic initiation factor 4G binding [GO:0031370];
poly(A RNA binding [GO:0044822];
repressing transcription factor binding [GO:0070491];
RNA cap binding [GO:0000339];
translation initiation factor activity [GO:0003743]
Gene Ontology
(Cellular Component)
Complete annatation
chromatoid body [GO:0033391];
cytoplasm [GO:0005737];
cytoplasmic mRNA processing body [GO:0000932];
cytoplasmic stress granule [GO:0010494];
cytosol [GO:0005829];
eukaryotic translation initiation factor 4F complex [GO:0016281];
extracellular exosome [GO:0070062];
mRNA cap binding complex [GO:0005845];
perinuclear region of cytoplasm [GO:0048471];
RISC complex [GO:0016442]
Protein-protein interaction108292
Phylogenetic treeP06730
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.777626116637781.31068807029955e-071.23214332479947e-06
AZA vs. DISU-0.08024468446670180.7516275754200080.976021571236163
AZA vs. IL70.3418026351772130.07655506713379420.773641548161711
AZA vs. SAHA-0.1079690088607980.6588903864742050.897305868831662
DISU vs. CD3-1.870555345545275.79008338830178e-075.92530325833408e-06
DISU vs. IL70.4133923395610210.1020446597060570.422616276117804
DISU vs. SAHA-0.02742893156724730.9252538740761770.982806795139101
DMSO vs. AZA-0.1142216905953350.4978639742623491
DMSO vs. CD3-1.902964763544688.31289181935091e-099.05742389096077e-08
DMSO vs. DISU-0.03583992006467150.8835329025771810.987583589107999
DMSO vs. IL70.4633077749442110.01036506471586180.211725407305715
DMSO vs. SAHA-0.001455635577007030.9950853010872130.998821704885362
HIV vs. Mock in Activation-0.1736500731641810.7799682341311110.999983755607037
HIV vs. Mock in Latency-0.1121581629923290.5004610458300470.999834320637052
IL7 vs. CD3-1.426640275388351.29125038381694e-059.67827798869856e-05
SAHA vs. CD3-1.911432346336271.64291900417446e-071.61716455864854e-06
SAHA vs. IL7-0.4541270582184120.06312942339809880.214238836450793
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.7 4.56E-05 1.2 0.034930245 1.3 0.021495733
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.495651 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.978 0.904 0.961 0.903 0.875
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01649 7-Methyl-Gpppa experimental unknown unknown
DB01960 7n-Methyl-8-Hydroguanosine-5&,39;-Diphosphate experimental unknown unknown
DB02716 7-Methyl-Guanosine-5&,39;-Triphosphate experimental unknown unknown
DB05165 LY2275796 investigational unknown unknown
DB08217 2,2,5,5-TETRAMETHYL-3-(SULFANYLMETHYL)-2,5-DIHYDRO-1H-PYRROL-1-OL experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1IPB X-ray 2.0Å A=1-217.
1IPC X-ray 2.0Å A=1-217.
1WKW X-ray 2.1Å A=27-217.
2GPQ NMR - A=1-217.
2V8W X-ray 2.3Å A/E=1-217.
2V8X X-ray 2.3Å A/E=1-217.
2V8Y X-ray 2.1Å A/E=1-217.
2W97 X-ray 2.2Å A/B=1-217.
3AM7 X-ray 2.2Å A=27-217.
3TF2 X-ray 2.1Å A/B/C/D=1-217.
3U7X X-ray 2.1Å A/B=1-217.
4AZA X-ray 2.1Å A/C=1-217.
4BEA X-ray 2.5Å A=1-217.
4DT6 X-ray 2.6Å A=1-217.
4DUM X-ray 2.9Å A=1-217.
4TPW X-ray 1.5Å A/B=28-217.
4TQB X-ray 1.5Å A/B=28-217.
4TQC X-ray 1.8Å A/B=28-217.
4UED X-ray 1.7Å A=36-217.
5ABI X-ray 1.9Å A=23-217.
5EHC X-ray 2.4Å A=1-217.
5EI3 X-ray 1.7Å A=1-217.
5EIR X-ray 2.6Å A=1-217.
5EKV X-ray 3.6Å A/C=1-217.
5GW6 X-ray 1.9Å A=23-217.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr downregulates 22457629
Pr55(Gag) regulated by 22759308
Envelope surface glycoprotein gp120 upregulates 12089333

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa01521 EGFR tyrosine kinase inhibitor resistance - Homo sapiens (human)
hsa03013 RNA transport - Homo sapiens (human)
hsa04066 HIF-1 signaling pathway - Homo sapiens (human)
hsa04150 mTOR signaling pathway - Homo sapiens (human)
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa04211 Longevity regulating pathway - Homo sapiens (human)
hsa04910 Insulin signaling pathway - Homo sapiens (human)