Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000928
UniProt IDP23588
Primary gene name(s)EIF4B
Synonym gene name(s)unknown
Protein nameEukaryotic translation initiation factor 4B
Protein functionRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P23588
Gene Ontology
(Biological Process)
Complete annatation
cytoplasmic translation [GO:0002181];
eukaryotic translation initiation factor 4F complex assembly [GO:0097010];
formation of translation preinitiation complex [GO:0001731];
nuclear-transcribed mRNA poly(A tail shortening [GO:0000289];
regulation of translational initiation [GO:0006446];
translational initiation [GO:0006413]
Gene Ontology
(Molecular Function)
Complete annatation
helicase activity [GO:0004386];
nucleotide binding [GO:0000166];
poly(A RNA binding [GO:0044822];
ribosomal small subunit binding [GO:0043024];
RNA binding [GO:0003723];
RNA strand annealing activity [GO:0033592];
RNA strand-exchange activity [GO:0034057];
translation initiation factor activity [GO:0003743]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
eukaryotic translation initiation factor 4F complex [GO:0016281];
polysome [GO:0005844]
Protein-protein interaction108291
Phylogenetic treeP23588
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.4029423348412740.2184998024560660.326514874342438
AZA vs. DISU-0.08727543596171180.7303358826766650.974560663114398
AZA vs. IL70.01508681592383940.9372453048463360.999311006273513
AZA vs. SAHA0.1104250586822650.6497534437692650.892539734816999
DISU vs. CD30.3040932939447510.4049897619005860.537916615639213
DISU vs. IL70.09340421378022510.7102454608384330.925566244998217
DISU vs. SAHA0.1978115573616770.4977557081296150.823502821603777
DMSO vs. AZA-0.1347162027774120.4187880425398751
DMSO vs. CD30.256038891675450.4230005721918720.53740121606262
DMSO vs. DISU-0.04971138820923110.8386585894814150.982583769983649
DMSO vs. IL70.1571628278823490.3804752388810430.833558583708238
DMSO vs. SAHA0.2373984037176910.3126256399833130.663962137427825
HIV vs. Mock in Activation-0.01161366437592760.9850840402842140.999983755607037
HIV vs. Mock in Latency0.001341231512145740.9934786259888520.999834320637052
IL7 vs. CD30.4271202287298510.1853123107152850.304437325276456
SAHA vs. CD30.4872314680506620.1682830109764890.264062723635095
SAHA vs. IL70.0909133064612710.7083239658302580.866521300188712
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.4404 0.02237

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.830872 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.974 0.95 0.808 0.819 0.937
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
211937_at 1.58 No downregulated in CD8+ cells
211938_at 1.48 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1WI8 NMR - A=88-178.
2J76 NMR - E=77-176.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) incorporates 25631074

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03013 RNA transport - Homo sapiens (human)
hsa04150 mTOR signaling pathway - Homo sapiens (human)
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa05205 Proteoglycans in cancer - Homo sapiens (human)