Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000926
UniProt IDQ14240
Primary gene name(s)EIF4A2
Synonym gene name(s)DDX2B, EIF4F
Protein nameEukaryotic initiation factor 4A-II
Protein functionATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q14240
Gene Ontology
(Biological Process)
Complete annatation
negative regulation of RNA-directed RNA polymerase activity [GO:1900260];
nuclear-transcribed mRNA poly(A tail shortening [GO:0000289];
regulation of translational initiation [GO:0006446];
RNA secondary structure unwinding [GO:0010501];
translational initiation [GO:0006413];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
ATPase activity [GO:0016887];
ATP binding [GO:0005524];
ATP-dependent RNA helicase activity [GO:0004004];
helicase activity [GO:0004386];
poly(A RNA binding [GO:0044822];
translation initiation factor activity [GO:0003743]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
eukaryotic translation initiation factor 4F complex [GO:0016281];
perinuclear region of cytoplasm [GO:0048471]
Protein-protein interaction108290
Phylogenetic treeQ14240
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1605341298292720.6237898073529280.724829232628428
AZA vs. DISU0.0164998219964670.9479781323037740.996442780563503
AZA vs. IL70.09369134792162110.6245884148307030.999311006273513
AZA vs. SAHA-0.3508932752312790.1496955237007680.492795440983922
DISU vs. CD3-0.1570703916663290.6669191924080110.764206758370077
DISU vs. IL70.06826590738651040.7863880849081880.951256953219973
DISU vs. SAHA-0.3662489049525350.2087157044978440.582557504599146
DMSO vs. AZA0.007484050831517880.9641760328742531
DMSO vs. CD3-0.1653591713193460.604756551360710.699743275441559
DMSO vs. DISU-0.0110132439016410.9640179748491350.994294013509907
DMSO vs. IL70.0934866084823680.6015640126641150.912604767320708
DMSO vs. SAHA-0.3650400315468140.1210963476487290.415065511806684
HIV vs. Mock in Activation-0.05707670908597230.9267888441271780.999983755607037
HIV vs. Mock in Latency-0.08067159325251570.6231086489976650.999834320637052
IL7 vs. CD3-0.05879431762084340.8547104326934910.90666771103993
SAHA vs. CD3-0.5360990497492780.1306954523038920.215891753394678
SAHA vs. IL7-0.4483611737414080.06539341191952540.219199531081816
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
-1.4 0.072697102 -1.8 0.002050129 -2 0.03641624
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.235554 0.118286
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.008 1.054 1.068 1.107 1.125
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3BOR X-ray 1.8Å A=22-240.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin cleaves 22944692
Pr55(Gag) regulated by 22759308
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03013 RNA transport - Homo sapiens (human)